大疱性角膜病变患者角膜细胞中白细胞介素-1α、肿瘤坏死因子和血管内皮生长因子的表达

The expression of interleukin-1 alpha, TNF and VEGF in corneal cells of patients with bullous keratopathy.

作者信息

Grzetić-Lenac Renata, Merlak Maja, Balog Tihomir, Markusić Vedran, Dekaris Iva

机构信息

Department of Ophtalmology, Rijeka University Hospital Center, Rijeka, Croatia.

出版信息

Coll Antropol. 2011 Sep;35 Suppl 2:171-3.

PMID:22220428

Abstract

Bullous keratopathy (BK) is a chronic corneal edema with or without subepithelial bullae as a result of a loss of the endothelial cells. 15 patients with BK after cataract surgery with intraocular lens implantation, due to Fuchs dystrophy (n = 3) or corneal endothelial trauma (n = 12) were included in the study. All patients were treated by amniotic membrane transplantation (AMT). Corneal epithelial cells in patients suffering from BK secreted 3.91 +/- 3.09 pg/mL of IL-1 alpha, 4446 +/- 16.8 pg/mL of TNF and 81.43 +/- 37.81 pg/mL of VEGF-I. Levels of all 3 investigated cytokines were significantly higher as compared to controls (p < 0.005). Amniotic membranes that were used to treat investigated patients contained 638.98 +/- 613.98 pg/mL of IL-1ra, 0.026 +/- 0.009 pg/mL of sTNF and 81.39 +/- 21.01 pg/mL of VEGF-R. Beneficial clinical effect of the AMT in treating BK could be explained by its natural production of pro-inflammatory cytokine antagonists such as IL-ra, sTNF antagonist and VEGF-R.

摘要

大泡性角膜病变（BK）是一种由于内皮细胞丧失导致的伴有或不伴有上皮下大泡的慢性角膜水肿。本研究纳入了15例白内障手术后植入人工晶状体并发BK的患者，病因包括Fuchs营养不良（n = 3）或角膜内皮损伤（n = 12）。所有患者均接受了羊膜移植（AMT）治疗。BK患者的角膜上皮细胞分泌白细胞介素-1α（IL-1α）3.91±3.09 pg/mL、肿瘤坏死因子（TNF）4446±16.8 pg/mL和血管内皮生长因子-1（VEGF-I）81.43±37.81 pg/mL。与对照组相比，所有3种研究的细胞因子水平均显著升高（p < 0.005）。用于治疗研究患者的羊膜含有白细胞介素-1受体拮抗剂（IL-1ra）638.98±613.98 pg/mL、可溶性肿瘤坏死因子（sTNF）0.026±0.009 pg/mL和血管内皮生长因子受体（VEGF-R）81.39±21.01 pg/mL。AMT治疗BK的有益临床效果可以通过其天然产生促炎细胞因子拮抗剂来解释，如IL-ra、sTNF拮抗剂和VEGF-R。