College of Horticultural and Landscape Architecture, Southwest University, Chongqing, People's Republic of China.
Can J Microbiol. 2012 Jan;58(1):81-92. doi: 10.1139/w11-111. Epub 2012 Jan 5.
The arbuscular mycorrhizal (AM) fungal resources present in wheat fields of the Canadian Prairie were explored using 454 pyrosequencing. Of the 33 dominant AM fungal operational taxonomic units (OTUs) found in the 76 wheat fields surveyed at anthesis in 2009, 14 clustered as Funneliformis - Rhizophagus, 16 as Claroideoglomus, and 3 as Diversisporales. An OTU of Funneliformis mosseae and one OTU of Diversisporales each accounted for approximately 16% of all AM fungal OTUs. The former was ubiquitous, and the latter was mainly restricted to the Black and Dark Brown Chernozems. AM fungal OTU community composition was better explained by the Chernozem great groups (P = 0.044) than by measured soil properties. Fifty-two percent of the AM fungal OTUs were unrelated to measured soil properties. Black Chernozems hosted the largest AM fungal OTU diversity and almost twice the number of AM fungal sequences seen in Dark Brown Chernozems, the great group ranking second for AM fungal sequence abundance. Brown Chernozems hosted the lowest AM fungal abundance and an AM fungal diversity as low as that seen in Gray soils. We concluded that Black Chernozems are most conducive to AM fungal proliferation. AM fungi are generally distributed according to Chernozem great groups in the Canadian Prairie, although some taxa are evenly distributed in all soil groups.
利用 454 焦磷酸测序技术探索了加拿大草原麦田中的丛枝菌根（AM）真菌资源。在 2009 年花期对 76 个麦田进行调查时，发现了 33 个优势 AM 真菌分类单元（OTU），其中 14 个聚类为泡囊丛枝菌根-球囊霉属，16 个聚类为胶膜菌属，3 个聚类为多样化孢囊霉目。一个 Funneliformis mosseae 的 OTU 和一个 Diversisporales 的 OTU 分别约占所有 AM 真菌 OTU 的 16%。前者无处不在，后者主要局限于黑色和暗棕色黑钙土。AM 真菌 OTU 群落组成受黑钙土大组（P = 0.044）的解释优于受测量土壤特性的解释。52%的 AM 真菌 OTU 与测量的土壤特性无关。黑钙土拥有最大的 AM 真菌 OTU 多样性，几乎是暗棕色黑钙土的两倍，黑钙土是 AM 真菌序列丰度排名第二的大组。棕色黑钙土拥有最低的 AM 真菌丰度和与灰色土壤一样低的 AM 真菌多样性。我们得出结论，黑钙土最有利于 AM 真菌的增殖。AM 真菌通常根据加拿大草原黑钙土大组分布，尽管有些类群在所有土壤组中均匀分布。
