Children's Hospital Medical Center, Department of Neurology, Cincinnati, OH 45229-3039, USA.
Headache. 2012 Jan;52(1):68-79. doi: 10.1111/j.1526-4610.2011.02049.x. Epub 2012 Jan 6.
Exacerbation of migraine with menses is common in adolescent girls and women with migraine, occurring in up to 60% of females with migraine. These migraines are oftentimes longer and more disabling and may be related to estrogen levels and hormonal fluctuations.
This study identifies the unique genomic expression pattern of menstrual-related migraine (MRM) in comparison to migraine occurring outside the menstrual period and headache-free controls.
Whole blood samples were obtained from female subjects having an acute migraine during their menstrual period (MRM) or outside of their menstrual period (non-MRM) and controls (C)--females having a menstrual period without any history of headache. The messenger RNA was isolated from these samples, and genomic profile was assessed. Affymetrix Human Exon ST 1.0 (Affymetrix, Santa Clara, CA, USA) arrays were used to examine the genomic expression pattern differences between these 3 groups.
Blood genomic expression patterns were obtained on 56 subjects (MRM = 18, non-MRM = 18, and controls = 20). Unique genomic expression patterns were observed for both MRM and non-MRM. For MRM, 77 genes were identified that were unique to MRM, while 61 genes were commonly expressed for MRM and non-MRM, and 127 genes appeared to have a unique expression pattern for non-MRM. In addition, there were 279 genes that differentially expressed for MRM compared to non-MRM that were not differentially expressed for non-MRM. Gene ontology of these samples indicated many of these groups of genes were functionally related and included categories of immunomodulation/inflammation, mitochondrial function, and DNA homeostasis.
Blood genomic patterns can accurately differentiate MRM from non-MRM. These results indicate that MRM involves a unique molecular biology pathway that can be identified with a specific biomarker and suggest that individuals with MRM have a different underlying genetic etiology.
经前期和经期偏头痛在青少年女孩和偏头痛女性中很常见，多达 60%的偏头痛女性会出现这种情况。这些偏头痛往往持续时间更长，致残率更高，可能与雌激素水平和激素波动有关。
本研究旨在确定与经期无关的偏头痛（MRM）与经期外偏头痛和无头痛对照者相比的独特基因组表达模式。
从在经期（MRM）或经期外（非 MRM）发生急性偏头痛的女性和无头痛史的女性（对照组 C）中采集全血样本。从这些样本中分离信使 RNA，并评估基因组谱。使用 Affymetrix Human Exon ST 1.0（Affymetrix，Santa Clara，CA，USA）阵列检查这 3 组之间的基因组表达模式差异。
对 56 名受试者（MRM = 18，非 MRM = 18，对照组 = 20）进行了血液基因组表达模式检测。MRM 和非 MRM 均观察到独特的基因组表达模式。对于 MRM，鉴定出 77 个仅在 MRM 中表达的基因，61 个基因在 MRM 和非 MRM 中共同表达，127 个基因在非 MRM 中似乎具有独特的表达模式。此外，有 279 个基因在 MRM 中与非 MRM 相比差异表达，但在非 MRM 中不差异表达。这些样本的基因本体论表明，这些基因群中的许多具有功能相关性，包括免疫调节/炎症、线粒体功能和 DNA 稳态等类别。
血液基因组模式可准确区分 MRM 与非 MRM。这些结果表明，MRM 涉及独特的分子生物学途径，可以通过特定的生物标志物来识别，并表明 MRM 患者具有不同的潜在遗传病因。
