Ocular Wound Healing and Therapeutics Laboratory, Singapore Eye Research Institute, Singapore.
Invest Ophthalmol Vis Sci. 2012 Feb 2;53(2):587-95. doi: 10.1167/iovs.11-7941.
To investigate the expression of SPARC and matrix metalloproteinases (MMPs) in normal conjunctiva and pterygium tissues.
This study involved paired control or uninvolved conjunctiva and pterygium tissue from 21 patients. Quantitative real-time PCR was performed to assess SPARC and MMP mRNA expression, whereas Western blot analysis was performed to assess SPARC protein levels in normal conjunctiva and pterygium tissue. Tissue localization of SPARC, extracellular matrix proteins, and MMPs were determined by immunofluorescence analyses.
SPARC transcript and protein levels were upregulated in pterygium compared with normal conjunctiva. Immunofluorescence analyses showed localization of SPARC to the epithelial basement membrane and stroma of normal conjunctiva tissue. Increased SPARC in the pterygium stroma colocalized partially with elevated collagen I, fibronectin, α-SMA, and MMP-3. SPARC and MMP-3 also colocalized in the pterygium epithelium.
SPARC was upregulated in pterygium and may collaborate with increased MMP-3 in some patients to account for many of the phenotypic properties characteristic of pterygium.
研究 SPARC 和基质金属蛋白酶（MMPs）在正常结膜和翼状胬肉组织中的表达。
本研究涉及 21 名患者的配对对照或未受累的结膜和翼状胬肉组织。采用实时定量 PCR 评估 SPARC 和 MMP mRNA 表达，采用 Western blot 分析评估正常结膜和翼状胬肉组织中 SPARC 蛋白水平。通过免疫荧光分析确定 SPARC、细胞外基质蛋白和 MMPs 的组织定位。
与正常结膜相比，SPARC 转录本和蛋白水平在翼状胬肉中上调。免疫荧光分析显示 SPARC 定位于正常结膜组织的上皮基底膜和基质中。翼状胬肉基质中 SPARC 的增加部分与胶原 I、纤维连接蛋白、α-SMA 和 MMP-3 的升高共定位。SPARC 和 MMP-3 也在翼状胬肉上皮中共定位。
SPARC 在翼状胬肉中上调，并且可能与某些患者中 MMP-3 的增加协同作用，从而解释了翼状胬肉的许多表型特征。
