Craven Michael, Kotlikoff Michael I, Nadworny Alyson S
Biomedical Sciences Department, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
Methods Mol Biol. 2012;843:177-89. doi: 10.1007/978-1-61779-523-7_17.
Through directed differentiation of embryonic stem cells, it has been demonstrated that mesodermal lineages in the mammalian heart (smooth muscle, endothelial, and cardiac) develop from a common, multipotent cardiovascular precursor (Dev Biol 265:262-275, 2004; Cell 127:1137-1150, 2006; Dev Cell 11:723-732, 2006). Identification of cardiovascular precursor cells at various stages of lineage commitment has been determined by expression of multiple markers, including the stem cell factor receptor c-kit. Utilizing a bacterial artificial chromosome (BAC) transgenic mouse model in which EGFP expression is placed under control of the c-kit promoter (c-kit(BAC)-EGFP), work from our laboratory indicates that c-kit expression identifies a multipotent cardiovascular precursor cell population within the early postnatal heart that can be isolated, expanded, and differentiated in vitro into all three cell lineages that specify the heart (Proc Natl Acad Sci U S A 106:1808-1813, 2009).
通过对胚胎干细胞进行定向分化，已证明哺乳动物心脏中的中胚层谱系（平滑肌、内皮和心肌）起源于一种共同的多能心血管前体细胞（《发育生物学》265:262 - 275，2004；《细胞》127:1137 - 1150，2006；《发育细胞》11:723 - 732，2006）。通过多种标志物的表达确定了谱系定向不同阶段的心血管前体细胞，其中包括干细胞因子受体c - kit。利用一种细菌人工染色体（BAC）转基因小鼠模型，其中EGFP的表达受c - kit启动子控制（c - kit(BAC)-EGFP），我们实验室的研究表明，c - kit的表达可识别出生后早期心脏内的多能心血管前体细胞群体，该群体能够被分离、扩增，并在体外分化为构成心脏的所有三种细胞谱系（《美国国家科学院院刊》106:1808 - 1813，2009）。
