Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain.
Appl Microbiol Biotechnol. 2012 May;94(3):773-87. doi: 10.1007/s00253-011-3738-9. Epub 2012 Jan 6.
In the industrial yeast biomass production process, cells undergo an oxidative and other stresses which worsen the quality of the produced biomass. The overexpression of the thioredoxin codifying gene TRX2 in a wine Saccharomyces cerevisiae strain increases resistance to oxidative stress and industrial biomass production yield. We observed that variations in the TRX2 gene dose in wine yeast strains are relevant to determine the fermentative capacity throughout the industrial biomass production process. So, we studied the molecular changes using a transcriptomic approach under these conditions. The results provide an overview of the different metabolic pathways affected during industrial biomass production by TRX2 gene manipulation. The oxidative stress-related genes, like those related with the glutathione metabolism, presented outstanding variations. The data also allowed us to propose new thioredoxin targets in S. cerevisiae, such as hexokinase 2, with relevance for industrial fermentation performance.
在工业酵母生物量生产过程中，细胞会经历氧化等应激，这会降低所产生生物量的质量。在葡萄酒酵母菌株中过表达硫氧还蛋白编码基因 TRX2 会增加其对氧化应激的抗性和工业生物量的生产产量。我们观察到，葡萄酒酵母菌株中 TRX2 基因剂量的变化与确定整个工业生物量生产过程中的发酵能力有关。因此，我们在这些条件下使用转录组学方法研究了分子变化。结果提供了 TRX2 基因操作在工业生物量生产过程中影响不同代谢途径的概述。与氧化应激相关的基因，如与谷胱甘肽代谢相关的基因，表现出显著的变化。这些数据还使我们能够在酿酒酵母中提出新的硫氧还蛋白靶标，例如与工业发酵性能相关的己糖激酶 2。
