Department of Cell and Molecular Biology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Mol Biol Rep. 2012 May;39(5):5785-90. doi: 10.1007/s11033-011-1389-7. Epub 2012 Jan 6.
White tail disease (WTD) is a serious viral disease in the hatcheries and nursery ponds of Macrobrachium rosenbergii in many parts of the world. A new disease similar to WTD was observed in larvae and post larvae of M. rosenbergii cultured in Malaysia. In the present study, RT-PCR assay was used to detect the causative agents of WTD, M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) using specific primers for MrNV RNA2 and XSV. The results showed the presence of MrNV in the samples with or without signs of WTD. However, XSV was only detected in some of the MrNV-positive samples. Phylogenetic analysis showed that the RNA2 of our Malaysian isolates were significantly different from the other isolates. Histopathological studies revealed myofiber degeneration of the tail muscles and liquefactive myopathy in the infected prawns. This was the first report on the occurrence of MrNV in the Malaysian freshwater prawn.
白尾病（WTD）是世界上许多地区罗氏沼虾孵化场和育苗池中的一种严重病毒性疾病。在马来西亚养殖的罗氏沼虾幼虫和幼虾中观察到一种类似于 WTD 的新疾病。在本研究中，使用针对 MrNV RNA2 和 XSV 的特异性引物，通过 RT-PCR 检测法检测 WTD 的病原体罗氏沼虾诺达病毒（MrNV）和超小型病毒（XSV）。结果表明，有或没有 WTD 症状的样本中均存在 MrNV。然而，XSV 仅在一些 MrNV 阳性样本中检测到。系统进化分析表明，我们马来西亚分离株的 RNA2 与其他分离株有显著差异。组织病理学研究显示，感染对虾的尾部肌肉肌纤维变性和液化性肌病。这是马来西亚淡水虾发生 MrNV 的首次报告。
