Eastern Kentucky University, Department of Chemistry, 521 Lancaster Ave., Richmond, KY 40475, USA.
Rapid Commun Mass Spectrom. 2012 Feb 15;26(3):385-91. doi: 10.1002/rcm.5316.
Presented here are findings for the development and optimization of a simple, high-throughput, and rapid method for the analysis of glucose. Because the applications of glucose and other six-carbon sugars is a growing field of interest especially in the production of biofuels, an efficient and rapid method for their quantitation from lignocelluloses is necessary. Glucose was analyzed using direct analysis in real time (DART) ionization and formed adducts (along with fragmentation) were observed with a linear ion trap (LIT) mass spectrometer. Since DART can be considered a complex thermal desorption ionization process, an optimization study of the helium gas temperature and introduction into the ionization region was performed. It was observed these parameters have a significant effect on the overall signal intensity as well as the signal-to-noise ratios in DART mass spectra. Using these optimized parameters, a set of different glucose concentrations (ranging from 10 to 3000 μM) were analyzed and used to determine a linear dynamic range (with the use of an internal standard). The analysis of the samples was done with minimal sample preparation and found to be reproducible on different days.
本文介绍了一种简单、高通量、快速分析葡萄糖的方法的开发和优化。由于葡萄糖和其他六碳糖的应用是一个日益增长的研究领域，特别是在生物燃料的生产中，因此需要一种从木质纤维素中高效、快速定量它们的方法。使用实时直接分析 (DART) 离子化分析葡萄糖，并使用线性离子阱 (LIT) 质谱仪观察形成的加合物（以及碎片）。由于 DART 可以被认为是一种复杂的热解吸电离过程，因此对氦气温度和引入电离区的参数进行了优化研究。结果表明，这些参数对整体信号强度以及 DART 质谱中的信噪比有显著影响。使用这些优化参数，分析了一组不同浓度的葡萄糖（范围为 10 到 3000 μM），并使用内标确定了线性动态范围。对样品的分析只需进行最小的样品制备，并且在不同的日子里具有重现性。
