头颈部鳞状细胞癌患者手术深层切缘的分子绘图组织印片。
Tissue imprint for molecular mapping of deep surgical margins in patients with head and neck squamous cell carcinoma.
机构信息
Department of Otolaryngology - Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
出版信息
Head Neck. 2012 Nov;34(11):1529-36. doi: 10.1002/hed.21982. Epub 2012 Jan 3.
Abstract
BACKGROUND
Tissue imprinting can generate molecular marker maps of tumor cells at deep surgical margins. The purpose of this study was to evaluate the feasibility of this method for detection of residual head and neck squamous cell carcinoma (HNSCC).
METHODS
Paired fresh tissue and nitrocellulose membrane imprints of tumor and deep margins were collected from 17 HNSCC resections. DNA was amplified using quantitative methylation-specific polymerase chain reaction (qMSP) for p16, DCC, KIF1A, and EDNRB. Levels of methylation in tumors and deep margins were compared.
RESULTS
DNA from imprints was adequate for qMSP. Hypermethylation of target genes was present in 12 of 17 tumors and in 8 deep margins. Methylation level was better from margin imprints than tissue. During follow-up (median, 13 months), local or regional recurrences occurred in 6 cases of which 5 had molecularly positive margins.
CONCLUSION
Tissue imprinting is feasible for molecular detection of residual tumor at deep surgical margins and may correlate with locoregional recurrence.
摘要
背景
组织印迹技术可生成肿瘤细胞在深部手术切缘的分子标志物图谱。本研究旨在评估该方法检测头颈部鳞状细胞癌（HNSCC）残留的可行性。
方法
从 17 例 HNSCC 切除术中收集配对的新鲜组织和肿瘤及深部切缘的硝酸纤维素膜印迹。采用定量甲基化特异性聚合酶链反应（qMSP）对 p16、DCC、KIF1A 和 EDNRB 进行扩增。比较肿瘤和深部切缘中 DNA 甲基化水平。
结果
印迹的 DNA 适合 qMSP。17 例肿瘤中有 12 例和 8 例深部切缘存在靶基因的高甲基化。印迹边缘的甲基化水平优于组织。在随访期间（中位数 13 个月），6 例发生局部或区域性复发，其中 5 例分子切缘阳性。
结论
组织印迹技术可用于检测深部手术切缘残留肿瘤的分子标志物，且可能与局部区域复发相关。
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