Ferrali M, Ciccoli L, Signorini C, Comporti M
Istituto di Patologia Generale dell'Università di Siena, Italy.
Biochem Pharmacol. 1990 Oct 1;40(7):1485-90. doi: 10.1016/0006-2952(90)90444-p.
Allyl alcohol administration in a toxic dose (1.5 mmol/kg) to starved mice causes the development of hemolysis in nearly 50% of the animals. Malonic dialdehyde (MDA) appears in plasma of the animals showing hemolysis. The treatment of mice with desferrioxamine after allyl alcohol intoxication completely prevents lipid peroxidation and hemolysis, suggesting the involvement of iron in the allyl alcohol-induced erythrocyte damage. Erythrocytes obtained from intoxicated mice before the development of hemolysis show, upon incubation, release of iron, lipid peroxidation and lysis. Studies carried out with reconstituted systems of erythrocyte lysates, containing ghosts and different fractions of erythrocyte cytosol and incubated in the presence of acrolein (the major metabolite of allyl alcohol), strongly suggest that iron is released from hemoglobin. This iron appears to promote lipid peroxidation which is accompanied by erythrocyte lysis. Thus, the allyl alcohol-induced hemolysis appears to be a model for iron delocalization from iron stores.
给饥饿小鼠给予中毒剂量（1.5 mmol/kg）的烯丙醇，会导致近50%的动物发生溶血。丙二醛（MDA）出现在发生溶血的动物血浆中。烯丙醇中毒后用去铁胺治疗小鼠可完全预防脂质过氧化和溶血，这表明铁参与了烯丙醇诱导的红细胞损伤。在溶血发生前从中毒小鼠获得的红细胞，在孵育时会出现铁释放、脂质过氧化和裂解。用含有红细胞膜泡和不同红细胞胞质组分的红细胞裂解物重组系统进行的研究，在丙烯醛（烯丙醇的主要代谢产物）存在下孵育，强烈提示铁从血红蛋白中释放。这种铁似乎会促进脂质过氧化，进而伴随红细胞裂解。因此，烯丙醇诱导的溶血似乎是铁从铁储存中游离的一个模型。
