Swann J D, Acosta D
Division of Pharmacology/Toxicology, College of Pharmacy, University of Texas, Austin 78712.
Biochem Pharmacol. 1990 Oct 1;40(7):1523-6. doi: 10.1016/0006-2952(90)90449-u.
The role of lipid peroxidation in gentamicin cytotoxicity was assessed in primary cultures of renal cortical epithelial cells. The cellular content of malondialdehyde (MDA), one of the end products of lipid peroxidation, was determined, and the intracellular generation of reactive oxygen species, one of the events commonly occurring at the beginning of the process of lipid peroxidation, was estimated. Exposures to gentamicin were chosen that preceded or accompanied overt toxicity. MDA was determined by the thiobarbituric acid reactive substances assay. Intracellular generation of reactive oxygen species was estimated by quantitating the fluorescence of 2',7'-dichlorofluorescein (DCF'), a fluorophore formed by the reaction of the deacetylation product (2',7'-dichlorofluorescein, DCF) of the fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFDA) with reactive oxygen species. Neither elevated MDA content nor intracellular generation of reactive oxygen species was observed in primary cultures of renal cortical epithelial cells treated with gentamicin, using exposures which preceded or accompanied overt toxicity. We conclude that lipid peroxidation does not play a role in gentamicin cytotoxicity.
在肾皮质上皮细胞原代培养物中评估了脂质过氧化在庆大霉素细胞毒性中的作用。测定了脂质过氧化终产物之一丙二醛（MDA）的细胞含量，并估算了脂质过氧化过程开始时常见的事件之一活性氧的细胞内生成情况。选择在明显毒性出现之前或同时进行的庆大霉素暴露。通过硫代巴比妥酸反应性物质测定法测定MDA。通过定量荧光探针2',7'-二氯荧光素二乙酸酯（DCFDA）的脱乙酰化产物（2',7'-二氯荧光素，DCF）与活性氧反应形成的荧光团2',7'-二氯荧光素（DCF'）的荧光来估算活性氧的细胞内生成情况。在使用在明显毒性出现之前或同时进行的暴露处理的肾皮质上皮细胞原代培养物中，未观察到MDA含量升高或活性氧的细胞内生成。我们得出结论，脂质过氧化在庆大霉素细胞毒性中不起作用。
