Arbatskiĭ N P, Zheltova A O, Iurtov D V, Kharitonenkov I G, Abashev Iu P, Derevitskaia V A, Kochetkov N K
Bioorg Khim. 1990 Jun;16(6):801-7.
The main surface glycoprotein, hemagglutinin (HA), was obtained by treatment of influenza virus B/Leningrad/179/86 with bromelain. Amino acid and monosaccharide compositions of HA and neuraminidase (NA, earlier isolated from the same virus) were determined, thus showing HA and NA to contain 8-10 and 2 carbohydrate chains, respectively. The carbohydrate fragments were cleaved off by the alkaline LiBH4 treatment, the oligosaccharides released were reduced with NaB3H4 and fractionated by two-step HPLC on Ultrasphere-C18 and Zorbax-NH2 columns. Some higher mannose and complex oligosaccharides were identified in both cases by comparison with nonlabelled oligosaccharides of the known structure. The data obtained show that surface glycoproteins of influenza virus A and B are rather similar with regard to structure and heterogeneity of their carbohydrate chains.
主要表面糖蛋白血凝素（HA）是通过用菠萝蛋白酶处理B/列宁格勒/179/86流感病毒获得的。测定了HA和神经氨酸酶（NA，早期从同一病毒中分离）的氨基酸和单糖组成，结果表明HA和NA分别含有8 - 10条和2条碳水化合物链。通过碱性LiBH4处理去除碳水化合物片段，释放的寡糖用NaB3H4还原，并在Ultrasphere - C18和Zorbax - NH2柱上通过两步高效液相色谱法进行分级分离。通过与已知结构的未标记寡糖比较，在两种情况下都鉴定出了一些高甘露糖型和复合型寡糖。所获得的数据表明，甲型和乙型流感病毒的表面糖蛋白在碳水化合物链的结构和异质性方面相当相似。
