Norwegian Institute of Public Health, Division of Forensic Medicine and Drug Abuse Research, P.O. Box 4404, Nydalen, 0403 Oslo, Norway.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Feb 15;885-886:37-42. doi: 10.1016/j.jchromb.2011.12.009. Epub 2011 Dec 27.
The demand of high throughput methods for the determination of gamma-hydroxybutyrate (GHB) and its precursors gamma-butyrolactone (GBL) and 1,4-butane-diol (1,4BD) as well as for pregabalin is increasing. Here we present two analytical methods using ultra-high pressure liquid chromatography (UPLC) and tandem mass spectrometric (MS/MS) detection for the determination of GHB, beta-hydroxybutyrate (BHB), pregabalin, 1,4BD and GBL in whole blood and urine. Using the 96-well formate, the whole blood method is a simple high-throughput method suitable for screening of large sample amounts. With an easy sample preparation for urine including only dilution and filtration of the sample, the method is suitable for fast screening of urine samples. Both methods showed acceptable linearity, acceptable limits of detection, and limits of quantification. The within-day and between-day precisions of all analytes were lower than 10% RSD. The analytes were extracted from matrices with recoveries near 100%, and no major matrix effects were observed. Both methods have been used as routine screening analyses of whole blood and urine samples since January 2010.
对于测定 γ-羟基丁酸 (GHB) 及其前体 γ-丁内酯 (GBL) 和 1,4-丁二醇 (1,4BD) 以及普瑞巴林的高通量方法的需求正在增加。在这里，我们介绍了两种使用超高效液相色谱 (UPLC) 和串联质谱 (MS/MS) 检测的分析方法，用于测定全血和尿液中的 GHB、β-羟基丁酸 (BHB)、普瑞巴林、1,4BD 和 GBL。使用 96 孔甲酸形式，全血方法是一种简单的高通量方法，适用于大量样本的筛选。尿液的样品制备简单，仅包括稀释和过滤样品，该方法适用于尿液样品的快速筛选。两种方法均表现出可接受的线性、可接受的检测限和定量限。所有分析物的日内和日间精密度均低于 10%RSD。分析物从基质中提取，回收率接近 100%，并且没有观察到主要的基质效应。自 2010 年 1 月以来，这两种方法一直被用作全血和尿液样本的常规筛选分析。
