Wilkinson Deepti S, Taylor Rebecca C, Dillin Andrew
Howard Hughes Medical Institute, Glenn Center for Aging Research, Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Methods Cell Biol. 2012;107:353-81. doi: 10.1016/B978-0-12-394620-1.00012-6.
This chapter is dedicated to the study of aging in Caenorhabditis elegans (C. elegans). The assays are divided into two sections. In the first section, we describe detailed protocols for performing life span analysis in solid and liquid medium. In the second section, we describe various assays for measuring age-related changes. Our laboratory has been involved in several fruitful collaborations with non-C. elegans researchers keen on testing a role for their favorite gene in modulating aging (Carrano et al., 2009; Dong et al., 2007; Raices et al., 2008; Wolff et al., 2006). But even with the guidance of trained worm biologists, this undertaking can be daunting. We hope that this chapter will serve as a worthy compendium for those researchers who may or may not have immediate access to laboratories studying C. elegans.
本章致力于研究秀丽隐杆线虫（C. elegans）的衰老。实验分为两个部分。在第一部分中，我们描述了在固体和液体培养基中进行寿命分析的详细方案。在第二部分中，我们描述了用于测量与年龄相关变化的各种实验。我们实验室与热衷于测试其喜爱基因在调节衰老中的作用的非秀丽隐杆线虫研究人员进行了几次富有成果的合作（Carrano等人，2009年；Dong等人，2007年；Raices等人，2008年；Wolff等人，2006年）。但即使有训练有素的线虫生物学家的指导，这项任务也可能令人生畏。我们希望本章能成为那些可能有或可能没有直接进入研究秀丽隐杆线虫实验室机会的研究人员的宝贵汇编。
