National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
J Chromatogr A. 2012 Feb 3;1223:126-30. doi: 10.1016/j.chroma.2011.12.050. Epub 2011 Dec 23.
Porous ceramic/agarose composite adsorbents were designed and prepared with silica ceramic beads and 4% agarose gel, and then functionalized with a special ligand carboxymethyl. A novel method was introduced to fabricating of the porous silica ceramic beads. The morphology of SEM shows a spherical shape and a porous structure of the ceramic beads. Nitrogen adsorption-desorption analysis gives an average pore size of 287.5 Å, a BET surface area of 29.33 m²/g and a porosity of 41.8%, respectively. Additionally, X-ray diffraction pattern indicates that the amorphous silica has been transformed into two crystal phases of quartz and cristobalite, leading to a porous and rigid skeleton and ensuring the application of the composite beads at high flow velocities. Lysozyme of hen egg-white with the activity of 12,700 U/mg was purified by the composite ion-exchanger in one step and the recovery and purification factor reaches 95.2% and 7.9, respectively.
多孔陶瓷/琼脂糖复合材料吸附剂是用硅胶陶瓷珠和 4%琼脂糖凝胶设计和制备的，然后用特殊配体羧甲基化功能化。介绍了一种制备多孔硅胶陶瓷珠的新方法。SEM 的形态表明陶瓷珠呈球形和多孔结构。氮气吸附-脱附分析分别给出了平均孔径为 287.5 Å、BET 比表面积为 29.33 m²/g 和孔隙率为 41.8%。此外，X 射线衍射图谱表明无定形二氧化硅已转化为石英和方石英两种晶相，形成多孔刚性骨架，确保了复合珠在高流速下的应用。用复合离子交换剂一步法从蛋清中提取溶菌酶，其活性为 12700 U/mg，回收率和纯化因子分别达到 95.2%和 7.9。
