Graduate School of Science and Engineering (Science Section) and Venture Business Laboratory, Ehime University, Matsuyama City, Japan.
Anal Biochem. 2012 Mar 1;422(1):55-7. doi: 10.1016/j.ab.2011.12.023. Epub 2011 Dec 16.
Avidin from egg white was migrated toward a cathode of nondenaturing electrophoresis and then immobilized on a polyvinylidene difluoride membrane. Adrenocorticotropic hormone (ACTH) was specifically captured after the biotinylated anti-ACTH antibody was bound to the membrane-immobilized avidin, and the captured ACTH was digested by the biotinylated trypsin on the membrane after extraction. The digested polypeptides from the ACTH were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). These results indicate that target substances can be specifically trapped and digested on membrane-immobilized avidin.
卵清白蛋白中的亲和素被迁移到非变性电泳的阴极，然后固定在聚偏二氟乙烯膜上。生物素化抗促肾上腺皮质激素（ACTH）抗体与膜固定的亲和素结合后，特异性捕获 ACTH，提取后，膜上的生物素化胰蛋白酶将捕获的 ACTH 消化。通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）分析 ACTH 被消化后的多肽。这些结果表明，目标物质可以在膜固定的亲和素上被特异性捕获和消化。
