Institute for Behavioral Genetics, University of Colorado, Boulder, USA.
J Gerontol A Biol Sci Med Sci. 2012 Jun;67(7):726-33. doi: 10.1093/gerona/glr225. Epub 2012 Jan 6.
The level of green fluorescent protein expression from an hsp-16.2-based transcriptional reporter predicts life span and thermotolerance in Caenorhabditis elegans. The initial report used a high-copy number reporter integrated into chromosome IV. There was concern that the life-span prediction power of this reporter was not attributable solely to hsp-16.2 output. Specifically, prediction power could stem from disruption of some critical piece of chromatin on chromosome IV by the gpIs1 insertion, a linked mutation from the process used to create the reporter, or from an artifact of transgene regulation (multicopy transgenes are subject to regulation by C elegans chromatin surveillance machinery). Here we determine if the ability to predict life span and thermotolerance is specific to the gpIs1 insertion or a general property of hsp-16.2-based reporters. New single-copy hsp-16.2-based reporters predict life span and thermotolerance. We conclude that prediction power of hsp-16.2-based transcriptional reporters is not an artifact of any specific transgene configuration or chromatin surveillance mechanism.
基于 hsp-16.2 的转录报告基因的绿色荧光蛋白表达水平可预测秀丽隐杆线虫的寿命和耐热性。最初的报告使用整合到染色体 IV 中的高拷贝数报告基因。有人担心，这种报告基因的寿命预测能力并非仅仅归因于 hsp-16.2 的表达。具体而言，预测能力可能源于 gpIs1 插入对染色体 IV 上某些关键染色质的破坏，gpIs1 插入是创建报告基因过程中的一个连锁突变，或者源于转基因调控的假象（多拷贝转基因受到秀丽隐杆线虫染色质监测机制的调控）。在这里，我们确定预测寿命和耐热性的能力是否特定于 gpIs1 插入或 hsp-16.2 报告基因的一般特性。新的单拷贝 hsp-16.2 报告基因可预测寿命和耐热性。我们得出结论，hsp-16.2 转录报告基因的预测能力不是任何特定转基因构型或染色质监测机制的假象。
