Dewar D, Graham D I, McCulloch J
Wellcome Surgical Institute, University of Glasgow, United Kingdom.
J Neural Transm Park Dis Dement Sect. 1990;2(2):129-37. doi: 10.1007/BF02260900.
Using both quantitative autoradiography in sections and a homogenate preparation assay, the distribution and density of 3H-ketanserin binding to 5 HT2 receptors was examined in frontal cortex and the hippocampal region from six control subjects and seven subjects who had dementia of the Alzheimer type (DAT). There was no difference between control and DAT subjects in the levels of ketanserin binding in any region of the frontal cortex or hippocampus determined by quantitative autoradiography or in parallel experiments using homogenate preparations (e.g. left frontal cortex, layer III; controls, 34.4 +/- 1.6 pmol/g, DAT, 37.1 +/- 4.6 pmol/g). In all of the DAT brains there were abundant neuritic plaques (e.g. superficial layers of left frontal cortex; 35 +/- 7 plaques/mm2), and a marked reduction of choline acetyltransferase activity, (by 30-60% relative to controls), in both frontal cortex and the hippocampus. Thus, despite the presence of morphological abnormalities and a loss of cholinergic function, two classic features of DAT, 5 HT2 receptor binding was unaltered in this group of DAT brains compared to controls.
运用切片定量放射自显影技术和匀浆制备测定法，对6名对照受试者和7名患阿尔茨海默型痴呆（DAT）受试者的额叶皮质和海马区中与5-羟色胺2（5-HT2）受体结合的3H-酮色林的分布及密度进行了检测。通过定量放射自显影技术或使用匀浆制剂的平行实验（例如左额叶皮质，III层；对照受试者，34.4±1.6 pmol/g，DAT受试者，37.1±4.6 pmol/g）测定发现，额叶皮质或海马区任何区域的酮色林结合水平在对照受试者和DAT受试者之间均无差异。在所有DAT受试者的大脑中均存在大量神经炎性斑块（例如左额叶皮质浅层；35±7个斑块/mm2），并且额叶皮质和海马区的胆碱乙酰转移酶活性均显著降低（相对于对照受试者降低30%-60%）。因此，尽管存在形态学异常以及胆碱能功能丧失这两个DAT的典型特征，但与对照受试者相比，这组DAT受试者大脑中的5-HT2受体结合未发生改变。
