Faculty of Environmental Science & Engineering, Kunming University of Science & Technology, Kunming 650093, China.
Environ Pollut. 2012 Feb;161:192-8. doi: 10.1016/j.envpol.2011.10.026. Epub 2011 Nov 22.
Ofloxacin (OFL) was used as a model antibiotic and the quenching of OFL fluorescence by DOM was examined with an emphasis on temperature-dependent quenching kinetics. OFL fluorescence intensity was corrected for inner filter and temperature effects. The kinetics data were fitted well using a two-compartment pseudo first-order kinetics model. Three quenching compartments were identified using this model, namely, a very fast quenching compartment (q(0)) and two pseudo first-order quenching compartments (q(1) + q(2)). The q(0) values had a positive relationship with temperature, while (q(1) + q(2)) were negatively related with temperature. In addition, OFL-DOM binding quantified by (q(1) + q(2)) was consistent with binding result obtained from dialysis equilibrium system. We concluded that q(0) was resulted from dynamic quenching, while (q(1) + q(2)) was attributed to static quenching. The dynamic quenching of OFL by DOM accounted for 30-90% to the overall quenching and thus was very significant.
氧氟沙星（OFL）被用作模型抗生素，并用其研究 DOM 对 OFL 荧光的猝灭作用，重点关注温度依赖的猝灭动力学。OFL 荧光强度校正了内滤和温度效应。动力学数据拟合得很好，使用了双室拟一级动力学模型。使用该模型确定了三个猝灭区室，即非常快速猝灭区室（q(0)）和两个拟一级猝灭区室（q(1) + q(2)）。q(0)值与温度呈正相关，而（q(1) + q(2)）与温度呈负相关。此外，通过（q(1) + q(2)）定量的 OFL-DOM 结合与透析平衡系统获得的结合结果一致。我们得出结论，q(0)是由动态猝灭引起的，而（q(1) + q(2)）归因于静态猝灭。DOM 对 OFL 的动态猝灭占总猝灭的 30-90%，因此非常显著。
