Research Institute for Physical-Chemical Medicine, Moscow, Russia.
Infect Genet Evol. 2012 Jun;12(4):838-45. doi: 10.1016/j.meegid.2011.12.013. Epub 2011 Dec 30.
Identification and typing of mycobacteria is very important for epidemiology, susceptibility testing and diagnostic purposes. This paper describes the development and validation of the alternative methods for species identification and typing of mycobacteria based on a matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-ToF MS). Altogether there were 383 clinical isolates analyzed which include 348 strains of Mycobacterium tuberculosis complex (MTBC) (342 strains of M. tuberculosis and 6 strains of M. bovis) and 35 strains of nontuberculous mycobacteria (NTM) represented by 16 different species. Direct bacterial profiling (DBP) by means of MALDI-ToF MS was carried out. Cluster analysis of DBP mass spectra divided them into two large separate groups corresponding to MTBC and NTM, and also demonstrated the possibility of isolate identification at the species level. Spoligotyping protocol based on mass spectrometry was developed and validated, it matched completely to classical spoligotyping data. Our results suggest that MALDI-ToF MS has potential as a rapid and reproducible platform for the identification and typing of Mycobacterium species.
分枝杆菌的鉴定和分型对于流行病学、药敏试验和诊断目的非常重要。本文描述了基于基质辅助激光解吸/电离飞行时间质谱（MALDI-ToF MS）的分枝杆菌种鉴定和分型替代方法的开发和验证。共有 383 株临床分离株进行了分析，其中包括 348 株结核分枝杆菌复合群（MTBC）（342 株结核分枝杆菌和 6 株牛分枝杆菌）和 35 株非结核分枝杆菌（NTM），代表 16 个不同的种。通过 MALDI-ToF MS 进行直接细菌分析（DBP）。DBP 质谱的聚类分析将它们分为两个大的独立组，分别对应于 MTBC 和 NTM，还证明了在种水平上进行分离鉴定的可能性。基于质谱的 spoligotyping 方案被开发和验证，它与经典 spoligotyping 数据完全匹配。我们的结果表明，MALDI-ToF MS 具有作为一种快速、可重复的分枝杆菌种鉴定和分型平台的潜力。
