Yamada H, Kuraishi R, Amikura R M, Nagano H
Department of Physiological Chemistry, Tokyo Metropolitan Institute of Medical Science, Japan.
Mol Reprod Dev. 1990 Aug;26(4):356-60. doi: 10.1002/mrd.1080260410.
When centriolar complex isolated from starfish spermatozoa was injected into starfish immature oocytes (fully grown, germinal vesicle stage) followed by treating the latter with 1-methyladenine, mitotic events such as condensation and division of chromosomes of the female pronucleus and cytokinesis following completion of meiosis were observed. No cortical reaction detected in the oocytes. Essentially the same was noted for mature oocytes (pronuclear stage) into which the centriolar complex had been injected. The oocytes that had received sperm tail fraction or buffer alone did not initiate cleavage. It would thus appear that sperm centriolar complex is significantly essential to the initiation of cleavage.
将从海星精子中分离出的中心粒复合体注射到海星未成熟卵母细胞（完全成熟、生发泡期）中，随后用1-甲基腺嘌呤处理后者，观察到有丝分裂事件，如雌原核染色体的凝聚和分裂以及减数分裂完成后的胞质分裂。在卵母细胞中未检测到皮质反应。对于注射了中心粒复合体的成熟卵母细胞（原核期），观察到的情况基本相同。接受精子尾部片段或仅接受缓冲液的卵母细胞未开始分裂。因此，精子中心粒复合体对于启动卵裂似乎至关重要。
