Nagai T, Moor R M
Department of Molecular Embryology, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, United Kingdom.
Mol Reprod Dev. 1990 Aug;26(4):377-82. doi: 10.1002/mrd.1080260413.
The consequences of interactions between porcine sperm, eggs, and oviduct cells before and during fertilization in vitro (IVF) has been examined with particular reference to the block to polyspermy. The pattern of polypeptides secreted by porcine oviduct epithelial cells has been determined and its effects on sperm both during pre-fertilization co-culture and during fertilization have been examined. In standard IVF procedures with no oviduct cell involvement, high rates of penetration (91%) were accompanied by equally high rates of multiple sperm penetration (91% of penetrated eggs). Fertilization on oviduct cell monolayers or a combination of 1 h co-culture of sperm and oviduct cells before the addition of in vitro matured oocytes did not reduce polyspermy. However, a sperm-oviduct cell co-culture period of 2.5 h followed by IVF on oviduct cells selectively reduced the rate of polyspermy by 40% and 50% in two separate series of trials (United Kingdom and Japan, respectively): Overall fertilization rates after this treatment were high (95% or 84%, respectively). A 3.5 h period of pre-fertilization co-culture further reduced polyspermy to only 14% of penetrated eggs, but this treatment was accompanied by a sharp drop in the fertilization rate from an overall mean of 88% for all other groups to 19% after 3.5 h co-culture.
在体外受精（IVF）之前及期间，猪精子、卵子和输卵管细胞之间相互作用的后果已被研究，尤其涉及多精受精阻断。已确定猪输卵管上皮细胞分泌的多肽模式，并研究了其在受精前共培养期间及受精期间对精子的影响。在不涉及输卵管细胞的标准IVF程序中，高穿透率（91%）伴随着同样高的多精子穿透率（91%的穿透卵子）。在输卵管细胞单层上进行受精，或在添加体外成熟卵母细胞之前将精子与输卵管细胞共培养1小时，均未降低多精受精率。然而，在两个独立的试验系列（分别来自英国和日本）中，精子与输卵管细胞共培养2.5小时，随后在输卵管细胞上进行IVF，选择性地将多精受精率降低了40%和50%：此处理后的总体受精率较高（分别为95%或84%）。受精前共培养3.5小时进一步将多精受精率降低至仅14%的穿透卵子，但此处理伴随着受精率从所有其他组的总体平均88%急剧下降至3.5小时共培养后的19%。
