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黄孢原毛平革菌的细胞和分子损伤由氧化型染发剂引起。

Cellular and molecular damage of Phanerochaete chrysosporium by the oxidation hair dyes.

机构信息

Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, 94 Weijin Road, Tianjin 300071, People's Republic of China.

出版信息

Environ Sci Pollut Res Int. 2012 Jul;19(6):2172-8. doi: 10.1007/s11356-011-0717-8. Epub 2012 Jan 10.

Abstract

INTRODUCTION

The toxic effect of the oxidation hair dyes on Phanerochaete chrysosporium was investigated by exposure of this fungus in a nitrogen-limited culture medium to various concentrations of the oxidation hair dyes.

RESULTS

The results showed that both the size and the dry weight of the mycelial pellets of P. chrysosporium could be reduced when the concentration of the oxidation hair dyes was higher than 300 mg/L. By using the AFLP analysis and the UPGMA dendrogram, the DNA damage of P. chrysosporium by the oxidation hair dyes was also detected. Comparing with that in the control, the percent polymorphism under different concentrations of the oxidation hair dyes increased. In the meantime, the DNA similarity was decreased, which meant that the DNA damage was aggravated with an increase in the concentrations of the oxidation hair dyes.

CONCLUSION

Thus, as an environmental pollutant, the oxidation hair dyes have a toxic effect on P. chrysosporium at both cellular and molecular levels.

摘要

简介

本研究通过在氮限制培养基中暴露于不同浓度的氧化染发剂,考察了氧化染发剂对黄孢原毛平革菌的毒性作用。

结果

结果表明,当氧化染发剂的浓度高于 300mg/L 时,黄孢原毛平革菌的菌丝球的大小和干重都可以减少。通过 AFLP 分析和 UPGMA 聚类图,还检测到氧化染发剂对黄孢原毛平革菌的 DNA 损伤。与对照组相比,不同浓度氧化染发剂下的多态性百分比增加。同时,DNA 相似性降低,这意味着随着氧化染发剂浓度的增加,DNA 损伤加剧。

结论

因此,作为一种环境污染物,氧化染发剂在细胞和分子水平上对黄孢原毛平革菌都具有毒性作用。

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