College of Chemistry and Environment Protection Engineering, Southwest Minzu University, Chengdu 610041, China.
College of Chemistry and Environment Protection Engineering, Southwest Minzu University, Chengdu 610041, China.
Ecotoxicol Environ Saf. 2018 Oct 30;162:225-234. doi: 10.1016/j.ecoenv.2018.07.011. Epub 2018 Jul 11.
Carbon nanotubes (CNTs) are widely used in diverse areas with increasing annual production, thus the environmental impact of CNTs needs thorough investigation. In this study, we evaluated the effect of pristine multi-walled CNTs (p-MWCNTs) and oxidized multi-walled CNTs (o-MWCNTs) on white rot fungus Phanerochaete chrysosporium, which is the decomposer in carbon cycle and also has many applications in environmental remediation. Both p-MWCNTs and o-MWCNTs had no influence on the dry weight increase of P. chrysosporium and the pH value of culture system. The fibrous structure of P. chrysosporium was disturbed by p-MWCNTs seriously, while o-MWCNTs had litter influence. The ultrastructural changes were more evident for P. chrysosporium exposed to p-MWCNTs and only p-MWCNTs could penetrate into the cell plasma. The chemical composition of P. chrysosporium was nearly unchanged according to the infrared spectra. The laccase activity was suppressed by p-MWCNTs, while o-MWCNTs showed stimulating effect. The decoloration of reactive brilliant red X-3B was not affected by both CNT samples. However, serious inhibition of wood degradation was observed in the p-MWCNTs exposed groups, suggesting the potential threat of CNTs to the decomposition of carbon cycle. The implication to the environmental risks and safe applications of carbon nanomaterials is discussed.
碳纳米管（CNTs）在不同领域中的应用日益广泛，年生产量也在不断增加，因此需要对 CNTs 的环境影响进行深入研究。在本研究中，我们评估了原始多壁碳纳米管（p-MWCNTs）和氧化多壁碳纳米管（o-MWCNTs）对白腐真菌糙皮侧耳（Phanerochaete chrysosporium）的影响。糙皮侧耳是碳循环中的分解者，在环境修复方面也有许多应用。p-MWCNTs 和 o-MWCNTs 均未影响糙皮侧耳的干重增加和培养体系的 pH 值。p-MWCNTs 严重干扰了糙皮侧耳的纤维结构，而 o-MWCNTs 的影响较小。超微结构的变化在 p-MWCNTs 暴露的糙皮侧耳中更为明显，且只有 p-MWCNTs 能够穿透细胞质。根据红外光谱，糙皮侧耳的化学成分几乎没有变化。p-MWCNTs 抑制漆酶活性，而 o-MWCNTs 则表现出刺激作用。两种 CNT 样品均未影响活性艳红 X-3B 的脱色。然而，在 p-MWCNTs 暴露组中观察到严重的木材降解抑制现象，表明 CNTs 可能对碳循环的分解造成潜在威胁。本文讨论了这一发现对碳纳米材料环境风险和安全应用的意义。
