Bayer E A, Wilchek M
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
J Mol Recognit. 1990 Jun;3(3):102-7. doi: 10.1002/jmr.300030303.
The avidin/biotin system was applied as a general mediator in the adsorption/desorption or immobilization of biologically active macromolecules to solid supports. In this context, model biotinylated proteins (lectins and antibodies) were attached to avidin-coupled Sepharose. As examples for affinity chromatography, peanut agglutinin and anti-transferrin antibody were used to isolate asialofetuin and transferrin, respectively. The capacity and product yields were significantly better than those achieved with conventional affinity chromatography on CNBr-activated Sepharose columns containing the same lectin or antibody. Moreover, the columns were characterized by improved stability properties exhibiting remarkably low levels of leakage.
抗生物素蛋白/生物素系统作为一种通用介质，用于生物活性大分子在固体载体上的吸附/解吸或固定。在此背景下，将模型生物素化蛋白（凝集素和抗体）连接到抗生物素蛋白偶联的琼脂糖上。作为亲和色谱的示例，分别使用花生凝集素和抗转铁蛋白抗体来分离去唾液酸胎球蛋白和转铁蛋白。其容量和产物产率明显优于在含有相同凝集素或抗体的溴化氰活化琼脂糖柱上进行的传统亲和色谱。此外，这些柱子的稳定性得到改善，泄漏水平极低。
