Pauza C D, Singh M K
Developmental Biology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92138-9216.
AIDS Res Hum Retroviruses. 1990 Aug;6(8):1027-30. doi: 10.1089/aid.1990.6.1027.
Persistent human immunodeficiency virus type 1 (HIV-1) infection of U937 monocytic cells resulted in the accumulation of novel forms of extrachromosomal viral DNA. These DNA species are larger than the genome size of HIV-1 and persist indefinitely. The extrachromosomal viral DNA species (E-DNA) were shown to be structurally stable by subcloning of infected cell lines and restriction fragment analysis. Similar E-DNA structures were observed in independent infections. Persistently infected monocytic cells had low levels of viral antigens, reflecting the low levels of viral RNA that were detected. These results support a role for E-DNA in persistent HIV-1 infection of monocytic cells.
U937单核细胞持续感染1型人类免疫缺陷病毒（HIV-1）导致了新型染色体外病毒DNA的积累。这些DNA种类大于HIV-1的基因组大小，且能无限期持续存在。通过对感染细胞系进行亚克隆和限制性片段分析，表明染色体外病毒DNA种类（E-DNA）在结构上是稳定的。在独立感染中观察到了类似的E-DNA结构。持续感染的单核细胞中病毒抗原水平较低，这反映了所检测到的病毒RNA水平较低。这些结果支持E-DNA在单核细胞的HIV-1持续感染中发挥作用。
