Lebedeva L I, Dubatolova T D, Omel'ianchuk L V
Tsitologiia. 2011;53(10):788-92.
Changes of nuclear texture in mitotic cells of Drosophila melanogaster imaginal discs were studied. The distribution of voxels DAPI fluorescence intensities was used as the quantitative measure of the nuclear texture. The integral characteristics such as the portion of voxels with a given fluorescent signal level and autocorrelation of pixel intensities were used. We showed the nuclear texture has specific changes at different mitotic stages and this can be used for more precise staging of mitosis. Colchicines treatment pathologies, connected to abnormal mitoses, by nuclear-texture approach.
研究了黑腹果蝇成虫盘有丝分裂细胞中核纹理的变化。体素DAPI荧光强度的分布被用作核纹理的定量测量。使用了诸如具有给定荧光信号水平的体素部分和像素强度自相关等积分特征。我们表明，核纹理在不同的有丝分裂阶段有特定变化，这可用于更精确地划分有丝分裂阶段。通过核纹理方法研究与异常有丝分裂相关的秋水仙碱治疗病理学。
