Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.
Immunopharmacol Immunotoxicol. 2012 Aug;34(4):633-8. doi: 10.3109/08923973.2011.648266. Epub 2012 Jan 11.
Apocynin is a widely used antioxidant in both basic and clinical research. The current study aimed to investigate the protective effect of apocynin in an established alcoholic steatohepatitis rat model. Healthy SD rats were gastrically fed with ethanol (4.0 g/kg) for 8 weeks to induce alcoholic steatohepatitis. After 8 weeks, rats were fed with ethanol for another 2 weeks with or without a daily intraperitoneal injection of 25 mg/kg apocynin. After sacrificing, serum and liver samples were subjected to hepatic injury measurements. After 8-week ethanol induction, rats exhibited typical alcoholic steatohepatitis features including reduced body weight, hepatic histological changes, elevated serum alanine transaminase (ALT) level, increased levels of oxidative stress and inflammatory markers, NADPH oxidases, and renin-angiotensin system (RAS) marker. Co-treatment of apocynin alleviated the hepatic injury and biochemical parameters induced by alcoholic steatohepatitis. In conclusion, addition of apocynin significantly attenuates hepatic oxidative stress and inflammation induced by alcoholic steatohepatitis. This effect is partly through the inhibition of the RAS system.
白杨素是基础和临床研究中广泛使用的抗氧化剂。本研究旨在探讨白杨素在已建立的酒精性脂肪性肝炎大鼠模型中的保护作用。健康的 SD 大鼠经胃给予 4.0 g/kg 的乙醇 8 周，以诱导酒精性脂肪性肝炎。8 周后，大鼠继续用乙醇喂养 2 周，同时或不伴有每日腹腔注射 25mg/kg 白杨素。处死大鼠后，取血清和肝组织样本进行肝损伤测定。经过 8 周的乙醇诱导，大鼠表现出典型的酒精性脂肪性肝炎特征，包括体重减轻、肝组织学改变、血清丙氨酸转氨酶（ALT）水平升高、氧化应激和炎症标志物、NADPH 氧化酶和肾素-血管紧张素系统（RAS）标志物增加。白杨素的联合治疗减轻了酒精性脂肪性肝炎引起的肝损伤和生化参数变化。总之，白杨素显著减轻了酒精性脂肪性肝炎引起的肝氧化应激和炎症。这种作用部分是通过抑制 RAS 系统实现的。
