Evans A J, Perry M M, Gilbert A B
Biochim Biophys Acta. 1979 Apr 27;573(1):184-95. doi: 10.1016/0005-2760(79)90185-1.
The isolated basal lamina from the granulosa layer in ovarian follicles of the domestic fowl contains an abundance of spherical particles with a modal cross-sectional diameter of 25-30 nm. The lipid in this basal lamina is predominantly triacylglycerol and its total fatty acid composition resembles that of plasma very low density lipoprotein (VLDL). Immunodiffusion studies and immunoelectrophoresis indicated that this basal lamina contains diffusible antigen identifiable with plasma VLDL. Perfusion with an alkaline buffer displaced the particles from the basal lamina and subsequent perfusion with plasma VLDL in an acidic buffer resulted in the reappearance of particles of similar size and form. Alternatively, when the perfused basal lamina was subsequently perfused with VLDL-free serum, few particulate structures were observed. Measurements of total and VLDL triacylglycerol together with electron microscope studies of the untreated and of the perfused basal lamina provided further evidence for the identification of the majority of particles with plasma VLDL. Other particulate lipoprotein is most probably plasma low density lipoprotein (LDL). These studies demonstrated that this basal lamina is permeable to the circulating VLDL of the laying fowl.
从家鸡卵泡颗粒层分离出的基底层含有大量球形颗粒，其模态横截面直径为25 - 30纳米。该基底层中的脂质主要是三酰甘油，其总脂肪酸组成类似于血浆极低密度脂蛋白（VLDL）。免疫扩散研究和免疫电泳表明，该基底层含有可与血浆VLDL识别的可扩散抗原。用碱性缓冲液灌注可使颗粒从基底层中移出，随后在酸性缓冲液中用血浆VLDL灌注会导致出现大小和形态相似的颗粒。或者，当灌注后的基底层随后用无VLDL血清灌注时，观察到的颗粒结构很少。对总三酰甘油和VLDL三酰甘油的测量以及对未处理和灌注后的基底层的电子显微镜研究为将大多数颗粒鉴定为血浆VLDL提供了进一步证据。其他颗粒状脂蛋白很可能是血浆低密度脂蛋白（LDL）。这些研究表明，该基底层对产蛋鸡循环中的VLDL具有通透性。
