Key Laboratory of Cell Biology of China Ministry of Public Health, Department of Stem Cells and Regenerative Medicine, China Medical University, Shen Yang, Peoples Republic of China.
Cell Biol Int. 2012 Apr 1;36(4):415-8. doi: 10.1042/CBI20110173.
UCB (umbilical cord blood) as a resource of MSCs (mesenchymal stem cells) is widely accepted, but the quantity and characteristics of UCB-MSCs from different gestational ages have not been well studied. We have quantified the number of MSCs in UCB at different gestational ages using a multi-colour flowcytometer and compared the cell proliferation rates of these UCB-MSCs. Defining MSCs as CD44+/CD105+/CD34-/CD45 population, their numbers declined in the UCB at the gestational age. Proliferation rates were significantly higher in UCB before term than at full term. Non-full term UCB samples may be a better source of MSCs.
UCB（脐带血）作为 MSCs（间充质干细胞）的来源已被广泛接受，但不同胎龄 UCB 中的 MSC 数量和特征尚未得到很好的研究。我们使用多色流式细胞仪对不同胎龄 UCB 中的 MSC 数量进行了定量，并比较了这些 UCB-MSCs 的细胞增殖率。将 MSC 定义为 CD44+/CD105+/CD34-/CD45 群体，它们的数量在 UCB 中随胎龄的增加而减少。在足月前，UCB 的增殖率明显高于足月时。非足月 UCB 样本可能是 MSC 的更好来源。
