Manimaran P, Ramkumar G, Mohan M, Mangrauthia S K, Padmakumari A P, Muthuraman P, Bentur J S, Viraktamath B C, Balachandran S M
Directorate of Rice Research, Biotechnology; Rajendranagar, Hyderabad, India.
GM Crops. 2011 Jun-Dec;2(3):135-7. doi: 10.4161/gmcr.2.3.19213. Epub 2011 Jun 1.
Bacillus thuringiensis (Bt), a gram positive soil bacteria was first identified and named by Japanese microbiologist Shigetane Ishiwata in 1901. During sporulation Bt produces proteinaceous parasporal crystal proteins called δ-endotoxins, or Cry proteins, which are insecticidal. Numerous Cry proteins have been isolated and characterized from different Bt strains with activity against insects, mites and nematodes. Sprayable formulations containing these Cry proteins as active ingredients have contributed significantly in the field of insect pest management. Since the first cloning of cry genes from Bt,1 scientists have successively demonstrated that plants could be genetically engineered to express these cry genes for the control of dreadful insect pests. Eventually, the first transgenic crop expressing Btcry1Ac gene in cotton was approved in 1996 for commercial cultivation in the USA to manage bollworms.
苏云金芽孢杆菌(Bt)是一种革兰氏阳性土壤细菌,于1901年由日本微生物学家石渡繁胤首次鉴定并命名。在芽孢形成过程中,Bt会产生称为δ-内毒素或Cry蛋白的蛋白质类伴孢晶体蛋白,这些蛋白具有杀虫活性。已经从不同的Bt菌株中分离并鉴定出许多具有抗昆虫、螨虫和线虫活性的Cry蛋白。含有这些Cry蛋白作为活性成分的可喷雾制剂在害虫管理领域做出了重大贡献。自首次从Bt中克隆出cry基因以来,科学家们相继证明可以对植物进行基因工程改造,使其表达这些cry基因以控制可怕的害虫。最终,1996年,第一种在美国获批用于商业种植以防治棉铃虫的转Bt cry1Ac基因棉花问世。