Université de Toulouse, Toulouse, France.
Water Sci Technol. 2012;65(2):289-95. doi: 10.2166/wst.2012.795.
The aim of the work was to quantify the influence of the simultaneous presence of flocs and granules in the nitrifying activity in a sequencing batch airlift reactor (SBAR). The nitrification rate and oxygen limitation of flocs, granules and hybrid sludge was investigated using respirometric assays at different dissolved oxygen concentrations. The spatial distribution of Ammonium Oxidizing Bacteria (AOB) and Nitrite Oxidizing Bacteria (NOB) was investigated using fluorescence in situ hybridization (FISH). Results showed that the nitrification rate was much less sensitive to oxygen limitation in systems containing a fraction of flocs than in pure granular sludge. Ammonium Oxidizing Bacteria (AOB) were found to be distributed in similar quantities in flocs and granules whereas the Nitrite Oxidizing Bacteria (NOB) were located preferentially in granules. This study showed that the presence of flocs with granules could increase the robustness of the process to transitory reductions of aeration.
本工作旨在量化在序批式气升式反应器（SBAR）中同时存在絮体和颗粒对硝化活性的影响。通过呼吸测定法在不同溶解氧浓度下研究了絮体、颗粒和混合污泥的硝化速率和氧限制。使用荧光原位杂交（FISH）研究了氨氧化菌（AOB）和亚硝酸盐氧化菌（NOB）的空间分布。结果表明，与纯颗粒污泥相比，含有絮体的系统中的硝化速率对氧限制的敏感性要低得多。发现氨氧化菌（AOB）在絮体和颗粒中的分布数量相似，而亚硝酸盐氧化菌（NOB）则优先存在于颗粒中。这项研究表明，絮体和颗粒的存在可以提高过程对曝气短暂减少的鲁棒性。
