Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.
Virol J. 2012 Jan 10;9:10. doi: 10.1186/1743-422X-9-10.
A functional interferon regulatory element (IRE) has been found in the EnhI/X promoter region of hepatitis B virus (HBV) genome. The purpose of this study is to compare the gene order of responder and non-responder to interferon therapy in patients with chronic hepatitis B (CHB), so as to evaluate the relationship between IRE mutation and the response to interferon treatment for CHB patients.
Synthetic therapeutic effect is divided into complete response (CR), partial response (PR) and non-response (NR). Among the 62 cases included in this study, 40 cases (64.5%) were in the response group (CR and PR) and 22 (35.5%) cases were in the NR group. Wild type sequence of HBV IRE TTTCACTTTC were found in 35 cases (56.5%), and five different IRE gene sequences. included TTTtACTTTC, TTTCAtTTTC, TTTtAtTTTC, TTTtACTTTt and cTTtACcTTC, were found in 22 cases (35.5%), 1 case (1.6%), 1 case (1.6%), 2 cases (3.2%) and 1 case (1.6%) respectively. There were 41.9%cases (26/62) with forth base C→T mutation, consisted of 32.5% (13/40) cases in response group and 59.1% (13/22) cases in NR group. Among the 35 cases with IRE sequences, there were 67.5% (27/40) cases in response group and 36.4% (8/22) in NR group, and the difference in IRE sequences between two groups was statistic significantly (P = 0.027). The result suggested that there is likely relationship between the forth base mutation (C→T) of IRE region and the response of HBV to Interferon therapy, and this mutation may partially decrease the inhibition effect of interferon on HBV.
The forth base C→T mutation in IRE element of HBV may partially influence the response of Interferon treatment in CHB patients.
乙型肝炎病毒（HBV）基因组 EnhI/X 启动子区域存在一个功能性干扰素调节元件（IRE）。本研究旨在比较干扰素治疗慢性乙型肝炎（CHB）患者应答者和无应答者的基因顺序，以评估 IRE 突变与 CHB 患者干扰素治疗反应之间的关系。
合成治疗效果分为完全应答（CR）、部分应答（PR）和无应答（NR）。在本研究纳入的 62 例患者中，40 例（64.5%）为应答组（CR 和 PR），22 例（35.5%）为 NR 组。在 35 例（56.5%）患者中发现 HBV IRE TTTCACTTTC 的野生型序列，在 22 例（35.5%）患者中发现了五种不同的 IRE 基因序列，包括 TTTtACTTTC、TTTCAtTTTC、TTTcAtTTTC、TTTcAtTTTC 和 cTTtACcTTC。1 例（1.6%）患者发现 TTTtACTTTt，2 例（3.2%）患者发现 TTTtACTTTt，1 例（1.6%）患者发现 cTTtACcTTC。在 62 例患者中，有 41.9%（26/62）患者发生了第四个碱基 C→T 突变，其中应答组占 32.5%（13/40），NR 组占 59.1%（13/22）。在 35 例具有 IRE 序列的患者中，应答组占 67.5%（27/40），NR 组占 36.4%（8/22），两组之间的 IRE 序列差异具有统计学意义（P=0.027）。结果表明，IRE 区域第四个碱基突变（C→T）与 HBV 对干扰素治疗的反应之间可能存在一定关系，这种突变可能部分降低了干扰素对 HBV 的抑制作用。
HBV IRE 元件中的第四个碱基 C→T 突变可能部分影响 CHB 患者干扰素治疗的反应。
