Diarra Birama, Yonli Albert Théophane, Sorgho Pegdwendé Abel, Compaore Tegwindé Rebeca, Ouattara Abdoul Karim, Zongo Wendpagnangdé Arsène, Tao Issoufou, Traore Lassina, Soubeiga Serge Théophile, Djigma Florencia Wendkuuni, Obiri-Yeboah Dorcas, Nagalo Bolni-Marius, Pietra Virginio, Sanogo Rokia, Simpore Jacques
Biomolecular Research Center Pietro Annigoni (CERBA), BP 364 Ouagadougou 01, Burkina Faso.
Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof Joseph KI-ZERBO, Burkina Faso; BP 7021 Ouagadougou 03.
Mediterr J Hematol Infect Dis. 2018 Jan 1;10(1):e2018007. doi: 10.4084/MJHID.2018.007. eCollection 2018.
The presence of HBV DNA in the liver (with detectable or undetectable HBV DNA in the serum) of individuals tested HBsAg negative by currently available assays is defined occult B Infection (OBI). It remains a potential transmission threat and risk to HBV chronic infection. The purpose of this study was to determine the OBI prevalence among HBsAg negative subjects and to characterize associated genotypes.
Blood samples of 219 HBsAg-negative subjects tested by ELISA were collected. HBV DNA was investigated in all samples. Viral loads were determined using quantitative real-time PCR. All samples were screened for HBV markers (anti-HBc, anti-HBe, HBsAg). The Pre-S/S region of the HBV genome was sequenced. The database was analyzed using the SPSS and Epi info software. Phylogenetic analysis was performed using the BioEdit and MEGA software.
Of the 219 samples, 20.1% were anti-HBc positive, 1.8% HBeAg and 22.8% were anti-HBe positive. Fifty-six (56) (25.6%) of the samples had a detectable HBV DNA and viral loads ranging from 4 IU/mL to 13.6 10 IU/mL. Sixteen of them (16/56) had a viral load < 200 IU/mL, resulting in an OBI prevalence of 7.3% (16/219) in our study. The remaining 40 subjects had viral loads > 200 IU/mL, resulting in a "false OBI" prevalence of 18.3% (40/219). HBV genotype E was predominant followed by the quasi-sub-genotype A3. A single "false OBI" strain had the characteristic mutation G145R. Other mutations were observed and all located in the major hydrophilic region (MHR) of the S gene.
The study reported a prevalence of 7.3% of occult hepatitis B infection. It confirms the predominance of genotype E and the existence of a subgroup of quasi-sub-genotype A3 of HBV in Burkina Faso. It further provides information on the presence of "false OBI." This study has found mutations in the major hydrophilic region (MHR) of the pre-S/S gene of HBV.
通过现有检测方法检测出HBsAg阴性的个体，其肝脏中存在HBV DNA（血清中HBV DNA可检测到或不可检测到）被定义为隐匿性乙型肝炎感染（OBI）。它仍然是HBV慢性感染的一个潜在传播威胁和风险。本研究的目的是确定HBsAg阴性受试者中的OBI患病率，并对相关基因型进行特征分析。
收集219名通过ELISA检测为HBsAg阴性受试者的血样。对所有样本进行HBV DNA检测。使用定量实时PCR测定病毒载量。对所有样本进行HBV标志物（抗-HBc、抗-HBe、HBsAg）筛查。对HBV基因组的前S/S区域进行测序。使用SPSS和Epi info软件对数据库进行分析。使用BioEdit和MEGA软件进行系统发育分析。
在219个样本中，20.1%抗-HBc阳性，1.8%HBeAg阳性，22.8%抗-HBe阳性。56个（25.6%）样本的HBV DNA可检测到，病毒载量范围为4 IU/mL至13.6×10 IU/mL。其中16个（16/56）病毒载量<200 IU/mL，因此在我们的研究中OBI患病率为7.3%（16/219）。其余40名受试者病毒载量>200 IU/mL，“假OBI”患病率为18.3%（40/219）。HBV基因型E占主导地位，其次是准亚基因型A3。一个单一的“假OBI”毒株具有特征性突变G145R。还观察到其他突变，且均位于S基因的主要亲水区（MHR）。
该研究报告隐匿性乙型肝炎感染患病率为7.3%。它证实了基因型E的主导地位以及布基纳法索存在HBV准亚基因型A3的一个亚组。它进一步提供了关于“假OBI”存在的信息。本研究在HBV前S/S基因的主要亲水区（MHR）发现了突变。
