Sadeghian Mohammad Hadi, Ayatollahi Hossein, Keramati Mohammad Reza, Memar Bahram, Jamedar Saeed Amel, Avval Maryam Mohammadnia, Sheikhi Maryam, Shaghayegh Gohar
Department of Hematology and Blood Bank, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Indian J Pathol Microbiol. 2011 Oct-Dec;54(4):720-4. doi: 10.4103/0377-4929.91504.
Multiple myeloma is a conventional term for clonal proliferation of plasma cells. It is suggested that viruses may have a significant role in the pathogenesis of multiple myeloma.
The aim of this study was to evaluate whether there is an association between multiple myeloma and Epstein-Barr Virus (EBV) by the polymerase chain reaction (PCR) method.
This case-control study was performed on 60 paraffin-embedded bone marrow biopsies (30 multiple myeloma and 30 normal bone marrow specimens) in the molecular pathology section of our hospital. The patients and control groups were matched according to gender and age. Several sections were cut from each paraffin blocks, and then the deoxyribonucleic acid (DNA) was extracted by the non-heating extraction method. In the next step, PCR was carried out for detection of EBV genome and finally its products were analyzed by electrophoresis.
DNA of EBV was detected in 10 patients of the case group (5 males and 5 females) and 3 subjects (2 males and 1 female) of the control group. The Pearson chi-square test showed significant difference between case and control group (P=0.03) for detection of the EBV genome. In myeloma patients, the mean white blood cell count was 9.05 + 4.02 and 5.20 + 2.02 Χ 10(9) /L in EBV positive and negative groups, respectively and a significant difference count was seen for the WBC count.
Our results show an association between multiple myeloma and EBV infection.
多发性骨髓瘤是浆细胞克隆性增殖的传统术语。有研究表明病毒可能在多发性骨髓瘤的发病机制中起重要作用。
本研究旨在通过聚合酶链反应（PCR）方法评估多发性骨髓瘤与爱泼斯坦-巴尔病毒（EBV）之间是否存在关联。
本病例对照研究在我院分子病理科对60份石蜡包埋的骨髓活检标本（30例多发性骨髓瘤和30例正常骨髓标本）进行。患者和对照组根据性别和年龄进行匹配。从每个石蜡块上切取多个切片，然后采用非加热提取法提取脱氧核糖核酸（DNA）。下一步，进行PCR检测EBV基因组，最后通过电泳分析其产物。
病例组10例患者（5例男性和5例女性）和对照组3例受试者（2例男性和1例女性）检测到EBV的DNA。Pearson卡方检验显示病例组和对照组在检测EBV基因组方面存在显著差异（P = 0.03）。在骨髓瘤患者中，EBV阳性和阴性组的平均白细胞计数分别为9.05 + 4.02和5.20 + 2.02×10⁹/L，白细胞计数存在显著差异。
我们的结果表明多发性骨髓瘤与EBV感染之间存在关联。
