Cheng LiTing, Lin Wen-Hui, Wang Pei-Chyi, Tsai Ming-An, Ho Ping-Yueh, Hsu Jung-Pin, Chern Red-Shyong, Chen Shih-Chu
Graduate Institute of Animal Vaccine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Dis Aquat Organ. 2011 Nov 3;97(1):17-23. doi: 10.3354/dao02407.
Taura syndrome virus (TSV) has spread worldwide, causing significant economic losses since Taura syndrome was first described in Ecuador in 1992. To determine the prevalence and impact of TSV infection on the shrimp farming industry in Taiwan, Pacific white shrimp Litopenaeus vannamei B. were collected from 220 farms between 2004 and 2006 for viral detection by reverse transcription polymerase chain reaction. Data showed that the overall TSV prevalence rate was 20% (43/220 farms). Comparing shrimp growth stages, TSV prevalence rates were 4% for postlarvae, 24% for juveniles, 24% for subadults, 32% for adults, and 5% for brooders. Among TSV-positive farms, average infection incidence was 35% in postlarvae farms, 55% in juvenile farms, 39% in subadult farms, 31% in adult farms, and 20% in brooder farms. Notably, TSV was also detected in Exopalaemon orientis H. from 1 of 10 farms. Tail fans and appendages had red pigmentation, which is characteristic of TSV infection. Of shrimp with pathological lesions, 100% had lesions on tail fans, 88% on appendages, and 80% in gills. Sequence comparison using the TSV VP1 (structural protein) gene showed that 9 isolates from the farms had 92.3 to 99.5% nucleotide sequence identity with strains in the GenBank database from Taiwan (AF406789 and AY355310) and Venezuela (DQ212790). This is the first broad epidemiological study of TSV infection in L. vannamei in Taiwan.
桃拉综合征病毒（TSV）自1992年在厄瓜多尔首次被描述以来已在全球传播，造成了重大经济损失。为了确定TSV感染在台湾对虾养殖业的流行情况和影响，2004年至2006年间从220个养殖场采集了凡纳滨对虾，通过逆转录聚合酶链反应进行病毒检测。数据显示，TSV总体流行率为20%（43/220个养殖场）。比较对虾的生长阶段，仔虾的TSV流行率为4%，幼虾为24%，亚成虾为24%，成虾为32%，亲虾为5%。在TSV呈阳性的养殖场中，仔虾养殖场的平均感染发生率为35%，幼虾养殖场为55%，亚成虾养殖场为39%，成虾养殖场为31%，亲虾养殖场为20%。值得注意的是，在10个养殖场中的1个养殖场的东方白虾中也检测到了TSV。尾扇和附肢有红色色素沉着，这是TSV感染的特征。在有病理损伤的对虾中，100%的尾扇有损伤，88%的附肢有损伤，80%的鳃有损伤。使用TSV VP1（结构蛋白）基因进行序列比较表明，来自养殖场的9个分离株与台湾（AF406789和AY355310）和委内瑞拉（DQ212790）GenBank数据库中的菌株核苷酸序列同一性为92.3%至99.5%。这是台湾首次对凡纳滨对虾TSV感染进行的广泛流行病学研究。
