Markosian K A, Pogosian G G, Paĭtian N A, Nalbandian R M
Biokhimiia. 1979 May;44(5):844-50.
The effects of cyanide, thiocyanide, azide, nitrite, nitrate, ferricyanide, persulfate, sulfide and halogenides on the intensities of the EPR spectrum and the band of 825 nm of cardiac cutochrome oxidase were studied. It was shown that according to their action on the copper the anions may be classified into three groups: 1) anions inducing the reduction of the copper (CN-, CNS-, S2-) anions changing the environment of the copper (N3-, NO2-); 3) anions slightly interacting with the copper (NO3-, halogenides). The incubation of cytochrome oxidase with ferricyanide led to the formation of a free-radical component without causing any pronounced changes in the copper environment; however, treatment of the protein with persulfate was accompanied by an irreversible modification of the copper EPR spectrum.
研究了氰化物、硫氰化物、叠氮化物、亚硝酸盐、硝酸盐、铁氰化物、过硫酸盐、硫化物和卤化物对心脏细胞色素氧化酶电子顺磁共振谱强度和825nm波段的影响。结果表明，根据它们对铜的作用，阴离子可分为三组：1）诱导铜还原的阴离子（CN-、CNS-、S2-）；2）改变铜环境的阴离子（N3-、NO2-）；3）与铜相互作用较弱的阴离子（NO3-、卤化物）。细胞色素氧化酶与铁氰化物孵育导致形成自由基成分，而不会引起铜环境的任何明显变化；然而，用过硫酸盐处理蛋白质会伴随着铜电子顺磁共振谱的不可逆修饰。
