Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar-364002, Gujarat, India.
Inorg Chem. 2012 Feb 6;51(3):1769-77. doi: 10.1021/ic202073q. Epub 2012 Jan 11.
Newly synthesized rhodamine derivatives, L(1) and L(2), are found to bind specifically to Hg(2+) or Cr(3+) in presence of large excess of other competing ions with associated changes in their optical and fluorescence spectral behavior. These spectral changes are significant enough in the visible region of the spectrum and thus, allow the visual detection. For L(1), the detection limit is even lower than the permissible [Cr(3+)] or [Hg(2+)] in drinking water as per standard U.S. EPA norms; while the receptor, L2 could be used as a ratiometric sensor for detection of Cr(3+) and Hg(2+) based on the resonance energy transfer (RET) process involving the donor naphthalimide and the acceptor Cr(3+)/Hg(2+)-bound xanthene fragment. Studies reveal that these two reagents could be used for recognition and sensing of Hg(2+)/Cr(3+). Further, confocal laser microscopic studies confirmed that the reagent L(2) could also be used as an imaging probe for detection of uptake of these ions in A431 cells.
新合成的罗丹明衍生物 L(1)和 L(2)在存在大量其他竞争离子的情况下，与 Hg(2+)或 Cr(3+)特异性结合，其光学和荧光光谱行为发生相应变化。这些光谱变化在光谱的可见区域非常显著，因此可以进行目视检测。对于 L(1)，检测限甚至低于美国环保署标准规定的饮用水中允许的 [Cr(3+)]或 [Hg(2+)]；而受体 L2 可以用作基于共振能量转移 (RET)过程的 Cr(3+)和 Hg(2+)的比率传感器，该过程涉及供体萘酰亚胺和受体 Cr(3+)/Hg(2+)-结合的呫吨片段。研究表明，这两种试剂可用于识别和检测 Hg(2+)/Cr(3+)。此外，共聚焦激光显微镜研究证实，试剂 L(2)也可用于作为检测 A431 细胞中这些离子摄取的成像探针。
