White J G
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.
Br J Haematol. 1990 Sep;76(1):108-15. doi: 10.1111/j.1365-2141.1990.tb07844.x.
Fibrinogen coupled to colloidal gold (Fgn/Au) has been used to follow GPIIb-IIIa receptors on surface-activated platelets and to evaluate the effects of the anti-actin agent, cytochalasin B (CB), on the movement of receptor-ligand complexes. Fgn/Au binds diffusely to spread platelets, but is translocated rapidly from the peripheral margin to cell centres and to channels of the open canalicular system (OCS). CB added after spreading and incubation with Fgn/Au reverses the centripetal movement of receptor-ligand complexes. Fgn/Au particles move back toward the margins of spread cells and form patches. The patches of receptor-ligand complexes overlie segments of the OCS filled with Fgn/Au. Washing out the CB reverses the process and receptor-ligand complexes are again translocated toward platelet centres.
与胶体金偶联的纤维蛋白原（Fgn/Au）已被用于追踪表面活化血小板上的糖蛋白IIb-IIIa受体，并评估抗肌动蛋白药物细胞松弛素B（CB）对受体-配体复合物运动的影响。Fgn/Au可弥散性结合铺展的血小板，但会迅速从外周边缘转移至细胞中心以及开放小管系统（OCS）的通道。在铺展并与Fgn/Au孵育后添加CB，可逆转受体-配体复合物的向心运动。Fgn/Au颗粒向铺展细胞的边缘移动并形成斑块。受体-配体复合物斑块覆盖在充满Fgn/Au的OCS节段上。洗去CB可使该过程逆转，受体-配体复合物再次向血小板中心转移。
