College of Material Science and Technology, Nanjing University of Aeronautics and Astronautics, China.
Acta Biochim Biophys Sin (Shanghai). 2012 Mar;44(3):241-8. doi: 10.1093/abbs/gmr126. Epub 2012 Jan 10.
Mercaptopropionic acid (MPA) and cysteamine (Cys) capped CdTe quantum dots (QDs) were successfully prepared and used to investigate the combined influence of surface modification, size distribution, and interaction time on their cytotoxicity in human pancreatic carcinoma (PANC-1) cells. Results indicated that the smaller the size of MPA-CdTe QDs, the higher the cytotoxicity, which could be partly due to the difference of their distribution inside cells. Comparing with MPA-CdTe QDs, Cys-CdTe QDs had better cellular metabolizability and lower cytotoxicity. These QDs' cellular distribution and cytotoxicity were closely related to their interaction time with cells. Their cytotoxicity was found to be significantly enhanced with the increase of incubation time in medium. After QD treatments, the influence of recover time on the final cell viability was also dependent on the concentration and surface modification of QDs used in pretreatment. The combined influence of these factors discussed here might provide useful information for understanding and reducing the cytotoxicity of QDs in future biomedical applications.
巯基丙酸（MPA）和半胱胺（Cys）修饰的碲化镉量子点（QDs）被成功制备，并用于研究表面修饰、尺寸分布和相互作用时间对其在人胰腺癌细胞（PANC-1）中的细胞毒性的综合影响。结果表明，MPA-CdTe QDs 的尺寸越小，其细胞毒性越高，这可能部分归因于它们在细胞内分布的差异。与 MPA-CdTe QDs 相比，Cys-CdTe QDs 具有更好的细胞代谢能力和更低的细胞毒性。这些 QDs 的细胞分布和细胞毒性与其与细胞的相互作用时间密切相关。随着在培养基中孵育时间的增加，它们的细胞毒性显著增强。QD 处理后，恢复时间对最终细胞活力的影响也取决于预处理中使用的 QD 的浓度和表面修饰。这里讨论的这些因素的综合影响可能为未来生物医学应用中理解和降低 QDs 的细胞毒性提供有用信息。
