Gupta Nalini, Barwad Adarsh, Rajwanshi Arvind, Kochhar Rakesh
Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Acta Cytol. 2012;56(1):80-4. doi: 10.1159/000332909. Epub 2012 Jan 4.
Human papilloma virus (HPV) has been repeatedly found in esophageal carcinoma tissues. However, detection rates of HPV DNA in these tumors have varied markedly. Differences in detection methods, sample types and geographic regions of the sample origin have been suggested as potential causes of this discrepancy. This study was undertaken to analyze the prevalence of HPV in esophageal carcinoma.
HPV L1 DNA was evaluated in a total of 49 esophageal carcinoma samples, including 44 cases of squamous cell carcinoma (SCC) and 5 cases of adenocarcinoma. Seventeen control samples of esophageal brushings were also analyzed. The HPV L1 fragment was detected using MY09/MY11 primers.
In test samples, 17/49 (34.7%) were positive for HPV L1 and, in comparison, none of the control samples were positive. HPV DNA was identified in 17/37 (46%) cases of non-keratinizing SCC and was not identified in any case of esophageal keratinizing SCC and adenocarcinoma.
This study defines a significant association of HPV with esophageal non-keratinizing SCC. Our findings raise the possibility that HPV is involved in esophageal carcinogenesis, especially the non-keratinizing type of SCC. Further investigation with a larger sample size over broader geographic areas may be warranted.
人乳头瘤病毒（HPV）在食管癌组织中屡有发现。然而，这些肿瘤中HPV DNA的检出率差异显著。检测方法、样本类型及样本来源的地理区域差异被认为是造成这种差异的潜在原因。本研究旨在分析食管癌中HPV的流行情况。
对总共49例食管癌样本进行HPV L1 DNA评估，其中包括44例鳞状细胞癌（SCC）和5例腺癌。还分析了17份食管刷检对照样本。使用MY09/MY11引物检测HPV L1片段。
在检测样本中，17/49（34.7%）HPV L1呈阳性，相比之下，对照样本均为阴性。在17/37（46%）例非角化性SCC病例中检测到HPV DNA，而在任何食管角化性SCC和腺癌病例中均未检测到。
本研究确定了HPV与食管非角化性SCC之间存在显著关联。我们的研究结果提示HPV可能参与食管癌的发生，尤其是非角化性SCC类型。可能需要在更广泛的地理区域进行更大样本量的进一步研究。
