Haeri Hayedeh, Mardani Omid, Asadi-Amoli Fahimeh, Shahsiah Reza
Department of Pathology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pathology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Cancer Research Center, Tehran University of Medical Sciences, Tehran Iran.
Acta Med Iran. 2014;52(3):197-200.
Human papillomavirus (HPV) has also been suggested as an etiology of esophageal squamous cell carcinoma (SCC). The aim of this study was to investigate the prevalence of HPV infection in esophageal SCCs in our region with strict contamination control to prevent false positive results. Thirty cases of esophageal squamous cell carcinomas were chosen by simple random selection in a period of two years. PCR for target sequence of HPV L1 gene was performed on nucleic acid extracted from samples by means of GP5+/GP6+ primers. All tissue samples in both case and control groups were negative for HPV-DNA. Although the number of cases in this study was limited, the contribution of HPV in the substantial number of esophageal SCCs in our region is unlikely.
人乳头瘤病毒（HPV）也被认为是食管鳞状细胞癌（SCC）的病因之一。本研究的目的是在严格控制污染以防止假阳性结果的情况下，调查我们地区食管鳞状细胞癌中HPV感染的患病率。在两年时间内通过简单随机抽样选取了30例食管鳞状细胞癌病例。使用GP5+/GP6+引物对从样本中提取的核酸进行HPV L1基因靶序列的PCR检测。病例组和对照组的所有组织样本HPV-DNA均为阴性。尽管本研究中的病例数量有限，但HPV在我们地区大量食管鳞状细胞癌中的作用不太可能存在。
