College of Life Science, Zhejiang University, 310058, Hangzhou, Zhejiang Province, PR China.
J Hazard Mater. 2012 Feb 29;205-206:47-54. doi: 10.1016/j.jhazmat.2011.12.001. Epub 2011 Dec 8.
The ability of Pseudomonas sp. strain DY1 to adsorb Acid Black 172 was studied to determine the kinetics and mechanisms involved in biosorption of the dye. Kinetic data for adsorption fit a pseudo-second-order model. Increased initial dye concentration could significantly enhance the amount of dye adsorbed by heat-treated biomass in which the maximum amount of dye adsorbed was as high as 2.98 mmol/g biomass, whereas it had no significant influence on dye sorption by live biomass. As treated temperature increased, the biomass showed gradual increase of dye sorption ability. Experiments using potentiometric titration and Fourier transform infrared spectroscopy (FTIR) indicated that amine groups (NH2) played a prominent role in biosorption of Acid Black 172. Scanning electron microscopy (SEM), atomic force microscopy (AFM) and transmission electron microscopy (TEM) analysis indicated that heat treatment of the biomass increased the permeability of the cell walls and denatured the intracellular proteins. The results of biosorption experiments by different cell components confirmed that intracellular proteins contributed to the increased biosorption of Acid Black 172 by heat-treated biomass. The data suggest that biomass produced by this strain may have application for removal of metal-complex dyes from wastewater streams generated from the dye products industry.
研究了假单胞菌（Pseudomonas sp. strain DY1）对酸性黑 172 的吸附能力，以确定生物吸附染料涉及的动力学和机制。吸附的动力学数据符合拟二级模型。增加初始染料浓度可以显著提高经热处理的生物质吸附染料的量，其中吸附的最大染料量高达 2.98mmol/g 生物质，而对活生物质的染料吸附没有显著影响。随着处理温度的升高，生物质表现出逐渐增加的染料吸附能力。电位滴定和傅里叶变换红外光谱（FTIR）实验表明，胺基（NH2）在酸性黑 172 的生物吸附中起重要作用。扫描电子显微镜（SEM）、原子力显微镜（AFM）和透射电子显微镜（TEM）分析表明，生物质的热处理增加了细胞壁的通透性并使细胞内蛋白质变性。不同细胞成分的吸附实验结果证实，细胞内蛋白质有助于增加热处理生物质对酸性黑 172 的吸附。这些数据表明，该菌株产生的生物质可能可用于从染料产品工业产生的废水中去除金属络合染料。
