Ali Imran, Al-Othman Zeid A, Al-Za'abi Mohammed
Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi, 110025, India.
Biomed Chromatogr. 2012 Aug;26(8):1001-8. doi: 10.1002/bmc.2690. Epub 2012 Jan 12.
Superficially porous silica particles columns (SPSPCs) are manufactured by different companies. The most common have the brand names Halo, Ascentis Express and Kinetex. These columns provide super fast, sharp peaks and moderate sample loading and back pressure. These are available in different chemistries such as C₈, C₁₈, RP Amide and Hilic. Normally, the silica gel particles have 2.7 and 1.7 µm total and inner solid core diameters with 0.5 µm thick outer porous layer, 90 Å pore size and 150 m²/g surface area. They have been used for the separation and identification of low and high molecular weight compounds. The present article describes the state of the art of superficially porous silica particles based columns with special emphasis on their structures, mechanisms of separation, applications and comparison.
表面多孔硅胶颗粒柱（SPSPCs）由不同公司生产。最常见的品牌有Halo、Ascentis Express和Kinetex。这些色谱柱具有超快速、尖锐的峰形以及适度的进样量和背压。它们有不同的化学类型，如C₈、C₁₈、反相酰胺和亲水作用色谱。通常，硅胶颗粒的总直径和内部实心核直径分别为2.7和1.7 µm，外部多孔层厚度为0.5 µm，孔径为90 Å，表面积为150 m²/g。它们已用于分离和鉴定低分子量和高分子量化合物。本文描述了基于表面多孔硅胶颗粒的色谱柱的技术现状，特别强调了它们的结构、分离机制、应用和比较。
