IspG 催化的脱氧木酮糖磷酸途径中甲基赤藓醇磷酸的位置同位素交换：机制意义。
IspG-catalyzed positional isotopic exchange in methylerythritol cyclodiphosphate of the deoxyxylulose phosphate pathway: mechanistic implications.
机构信息
Department of Chemistry, Boston University, Boston, MA 02215, USA.
出版信息
Chembiochem. 2011 Mar 7;12(4):527-30. doi: 10.1002/cbic.201000716. Epub 2011 Jan 26.
Abstract
H(2)(18)O under the bridge: Recently, the deoxyxylulose phosphate (DXP) pathway was discovered to be a second pathway supplying isoprenoid biosynthetic precursors. One of steps is an IspG-catalyzed reductive deoxygenation of methylerythritol cyclodiphosphate (MEcPP) to 4-hydroxyl-3-methyl-2-(E)-1-diphosphate (HMBPP). Using [2-(13) C,(18) O]-MEcPP, we detected the positional isotopic exchange for the bridging oxygen in MEcPP.
摘要
H(2)(18)O 桥下水：最近，人们发现脱氧木酮糖磷酸（DXP）途径是提供异戊烯基生物合成前体的第二条途径。该途径的一个步骤是 IspG 催化的甲基赤藓醇磷酸环二磷酸（MEcPP）的还原脱羟化为 4-羟基-3-甲基-2-（E）-1-二磷酸（HMBPP）。使用 [2-(13)C，(18)O]-MEcPP，我们检测到 MEcPP 中桥氧的位置同位素交换。
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