Shalaby Mona A, Mohamed Mervat S, Mansour Marwa A, Abd El-Haffiz Al Shimaa L
Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University, Egypt.
Clin Lab. 2011;57(11-12):919-24.
Listeria monocytogenes has emerged as a significant food borne pathogen in recent decades. Polymerase chain reaction (PCR) is deemed to be more reliable than conventional methods of identification. This work aimed to evaluate the accuracy of PCR in comparison to conventional methods for the diagnosis of L. monocytegenes from different clinical specimens and food stuffs.
This study was conducted on 66 clinical specimens and 100 different food stuffs. On the basis of colonial morphology, Gram's stain, catalase test, haemolysis on sheep blood agar, and motility test, Listeria isolates were further identified to species level by 10300 API Listeria strips. PCR was done directly for all specimens to evaluate its accuracy in comparison to conventional methods of diagnosis.
A total of 5 (7.6%) same L. monocytogenes isolates were identified both by the conventional method and PCR in different clinical samples. However, PCR identified 6 (6%) L. monocytogenes isolates from food stuffs versus 4 (4%) isolates were identified by conventional methods.
PCR is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes either from clinical specimens or food stuffs.
近几十年来，单核细胞增生李斯特菌已成为一种重要的食源性病原体。聚合酶链反应（PCR）被认为比传统鉴定方法更可靠。这项工作旨在评估与传统方法相比，PCR用于诊断不同临床标本和食品中单核细胞增生李斯特菌的准确性。
本研究对66份临床标本和100种不同食品进行。根据菌落形态、革兰氏染色、过氧化氢酶试验、在羊血琼脂上的溶血情况和动力试验，通过10300 API李斯特菌鉴定条将李斯特菌分离株进一步鉴定到种水平。对所有标本直接进行PCR，以评估其与传统诊断方法相比的准确性。
在不同临床样本中，通过传统方法和PCR共鉴定出5株（7.6%）相同的单核细胞增生李斯特菌分离株。然而，PCR从食品中鉴定出6株（6%）单核细胞增生李斯特菌分离株，而传统方法鉴定出4株（4%）。
PCR是一种快速的方法，对从临床标本或食品中快速检测和鉴定单核细胞增生李斯特菌具有敏感性和特异性。
