Awodele Olufunsho, Olayemi Sunday O, Nwite Joseph A, Adeyemo Titilope A
Department of Pharmacology, College of Medicine, University of Lagos, Lagos, Nigeria.
J Infect Dev Ctries. 2012 Jan 12;6(1):79-85. doi: 10.3855/jidc.1906.
Among opportunistic pathogens associated with acquired immunodeficiency syndrome (AIDS), Mycobacterium tuberculosis is distinguished by its relative virulence and potential for person-to-person transmission. Oxidative stress is associated with TB infection with a high level in patients with HIV-TB co-infection. However, the comparative level of oxidative stress in HIV patients on highly active anti-retroviral therapy (HAART) and naïve (untreated) HIV patients is not clear.
This study is aimed to determine the level of oxidative stress and haematological parameters in HIV patients (naïve), HIV patients undergoing HAART, and HIV-TB co-infected patients. In total, 100 consenting subjects were recruited from the AIDS Prevention Iniative in Nigeria (APIN) Clinic. For controls, normal male healthy volunteers were recruited from the blood donor clinic and females from the APIN clinic staff members, both of the Lagos University Teaching Hospital. Measurements of antioxidant enzyme activity and lipid peroxidation were performed according to standard procedures. Haematological parameters were determined using a fully automated hematology analyzer.
Red blood cells significantly decreased (P ≤ 0.001) in all patients when compared with control subjects. The lipid peroxidation (MDA) was significantly high (P ≤ 0.05) in naive patients when compared to HIV patients on HAART. The decrease in the levels of GSH in both naive and HIV-TB co-infected patients (P ≤ 0.001) and HIV patients on HAART (P < 0.05) were significant when compared to control patients.
There is lower antioxidant level and higher MDA in naive and HIV-TB co-infected patients as compared to HIV patients on HAART and the seronegative patients.
在与获得性免疫缺陷综合征（艾滋病）相关的机会性病原体中，结核分枝杆菌因其相对较强的毒力和人际传播潜力而备受关注。氧化应激与结核病感染相关，在艾滋病毒与结核病合并感染的患者中水平较高。然而，接受高效抗逆转录病毒治疗（HAART）的艾滋病毒患者与未接受治疗的初治艾滋病毒患者的氧化应激相对水平尚不清楚。
本研究旨在确定初治艾滋病毒患者、接受HAART的艾滋病毒患者以及艾滋病毒与结核病合并感染患者的氧化应激水平和血液学参数。总共从尼日利亚艾滋病预防倡议（APIN）诊所招募了100名同意参与的受试者。作为对照，从拉各斯大学教学医院的献血诊所招募正常男性健康志愿者，从APIN诊所工作人员中招募女性。根据标准程序进行抗氧化酶活性和脂质过氧化的测量。使用全自动血液分析仪测定血液学参数。
与对照受试者相比，所有患者的红细胞均显著减少（P≤0.001）。与接受HAART的艾滋病毒患者相比，初治患者的脂质过氧化（丙二醛，MDA）显著升高（P≤0.05）。与对照患者相比，初治患者和艾滋病毒与结核病合并感染患者的谷胱甘肽（GSH）水平降低（P≤0.001），接受HAART的艾滋病毒患者的GSH水平降低（P<0.05），差异均具有统计学意义。
与接受HAART的艾滋病毒患者和血清阴性患者相比，初治患者以及艾滋病毒与结核病合并感染患者的抗氧化水平较低，MDA水平较高。
