Tertov V V, Sobenin I A, Gabbasov Z A, Popov E G, Orekhov A N
Biull Eksp Biol Med. 1990 Jul;110(7):34-6.
Spontaneous aggregation of glycosylated, desialated, oxidized and malondialdehyde modified low density lipoprotein (LDL) as well as LDL of coronary heart disease patients has been discovered using methods for determination of light transmission fluctuations in suspensions and gel filtration. At the same time; LDL of healthy donors failed to aggregate under conditions of cellular culture. On the other hand, human aortic cells from unaffected intima incubated with modified LDL, but not native LDL of healthy donors, showed a rise in esterified cholesterol levels. There was a strong correlation between the degree of LDL aggregation and intracellular cholesterol ester accumulation (r-0.86, p 0.001, n-21). Removal of aggregates by passing preparations through and 0.1 um filter significantly inhibited the accumulation of cholesterol esters. The obtained data point to the essential, if not decisive, role of LDL aggregation in the processes of lipid accumulation by intimal cells in vitro.
利用悬浮液透光率波动测定法和凝胶过滤法，已发现糖基化、去唾液酸、氧化和丙二醛修饰的低密度脂蛋白（LDL）以及冠心病患者的LDL会发生自发聚集。与此同时，健康供体的LDL在细胞培养条件下不会聚集。另一方面，来自未受影响内膜的人主动脉细胞与修饰的LDL而非健康供体的天然LDL孵育后，酯化胆固醇水平会升高。LDL聚集程度与细胞内胆固醇酯积累之间存在很强的相关性（r = 0.86，p < 0.001，n = 21）。通过0.1μm滤器过滤制剂以去除聚集体，可显著抑制胆固醇酯的积累。所获得的数据表明，LDL聚集在体外内膜细胞脂质积累过程中即使不是起决定性作用，也是起着重要作用。
