采用快原子轰击质谱法和选择离子监测技术对猪胰岛素和牛胰岛素的混合物进行分析。

Mixtures of porcine and bovine insulin analysed by fast atom bombardment mass spectrometry with selected ion monitoring.

作者信息

Bojesen G

机构信息

Department of Chemistry, Odense University, Denmark.

出版信息

Biomed Environ Mass Spectrom. 1990 Sep;19(9):541-4. doi: 10.1002/bms.1200190904.

DOI:10.1002/bms.1200190904

PMID:2224181

Abstract

Mixtures of bovine and porcine insulin have been analysed by fast atom bombardment mass spectrometry with selected ion monitoring of M + H ions at m/z 5733.9 (bovine) and 5778.0 (porcine). Porcine insulin could be detected in a mixture of 2% porcine and 98% bovine insulin and with bovine insulin as the minor component as little as 1% could be detected.

摘要

牛胰岛素和猪胰岛素的混合物已通过快速原子轰击质谱法进行分析，对质荷比为5733.9（牛胰岛素）和5778.0（猪胰岛素）的M + H离子进行选择离子监测。在含有2%猪胰岛素和98%牛胰岛素的混合物中可以检测到猪胰岛素，并且当牛胰岛素作为次要成分时，低至1%的猪胰岛素也能被检测到。