用快原子轰击质谱法鉴定牛角膜中的甲硫氨酸脑啡肽。
Identification of methionine enkephalin in the bovine cornea by fast atom bombardment-mass spectrometry.
作者信息
Tinsley P W, Dass C, Trimble J W, Desiderio D M
机构信息
Charles B. Stout Neuroscience Mass Spectrometry Laboratory, Memphis, TN.
出版信息
Exp Eye Res. 1990 Dec;51(6):671-4. doi: 10.1016/0014-4835(90)90051-u.
PMID:2265678
Abstract
Native methionine enkephalin and methionine enkephalin generated proteolytically from a larger peptide were both identified and quantified from bovine cornea by negative ion fast atom bombardment-mass spectrometry. The corneal peptides were purified initially be reverses-phase high performance liquid chromatography and identified tentatively by radioreceptor assay and/or radioimmunoassay.
摘要
通过负离子快原子轰击质谱法，从牛角膜中鉴定并定量了天然甲硫氨酸脑啡肽以及由较大肽经蛋白水解产生的甲硫氨酸脑啡肽。角膜肽最初通过反相高效液相色谱法进行纯化，并通过放射受体测定和/或放射免疫测定法进行初步鉴定。
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